全蝎蜈蚣对CIA大鼠外周血CD4~+CD25~+FoxP3~+Treg细胞的调节

目的:观察全蝎蜈蚣对胶原免疫性关节炎(Collagen-Induced Arthritis,CIA)大鼠外周血CD4+CD25+FoxP3+Treg细胞的调节作用。方法:Wistar大鼠60只,随机分成6组,分别为正常对照组、模型对照组、全蝎蜈蚣高(0.4 g/kg)、中(0.2 g/kg)、低剂量(0.1 g/kg)组和Ⅱ型胶原蛋白(60μg/kg)治疗组(CⅡ组),二型胶原蛋白诱导大鼠关节炎,并采用流式细胞术检测大鼠外周血CD4+CD25+FoxP3+Treg细胞及酶联免疫吸附试验检测血清中IL-2表达水平。结果:与模型组比较,全蝎蜈蚣高、低剂量组大鼠外周血CD4+CD25+T细胞及CD4+CD25+FoxP3+Treg细胞水平显著升高,且中、低剂量组血清IL-2水平显著下降。结论:全蝎蜈蚣治疗关节炎大鼠可能是通过上调外周CD4+CD25+FoxP3+Treg细胞表达水平以恢复或重建免疫耐受而实现的。

Regulation Effect of Scorpio and Scolopendra on CD4~+CD25~+FoxP3~+Treg Cell in Peripheral Blood from Rats with Collagen-Induced Arthritis(CIA)

Objective:To study the regulation effect of Scorpio and Scolopendra on CD4+CD25+FoxP3+Treg Cell in peripheral blood from rats with collagen-induced arthritis.Methods:Sixty female Wistar rats were randomly divided into 6 groups,normal control group,model control group,low-dose,middle-dose and high-dose Scorpio and Scolopendra group,and the type Ⅱcollagen group.Rats′ arthritis was induced by collagen.The number of CD4+CD25+FoxP3+Treg cell in peripheral blood was tested by flow cytometry,and the level of IL-2 in serum was detected by enzyme linked immunosorbent assay.Results:Compared with the model groups,the levels of CD4+CD25+Treg cell and CD4+CD25+FoxP3+Treg cell were increased obviously in the high and low dose of Scorpio and Scolopendra groups(P<0.05 or P<0.01),while the level of IL-2 in serum was decreased remarkably in the middle and low dose of Scorpio and Scolopendra groups(P<0.05 or P<0.01).Conclusion:It is realized that Scorpio and Scolopendra effectively treat RA by regulating the level of CD4+CD25+FoxP3+Treg cell to restore immunological tolerance.