| Vascular Endothelial Growth Factorl65-regulated Nasopharyngeal Carcinoma Cell Lines Invasion and Migration Involve Expression and Activation of Matrix Metalloproteinase-2 |
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| 作者姓名： | 王彦君 孔维佳 乐建新 孙大为 李伟 姚琪 孙宇 董继华 |
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| 作者单位： | Department of Otorhinolaryngology，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022，China，Department，of Otorhinolaryngology，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022，China，Department，of Otorhinolaryngology，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022，China，Department，of Otorhinolaryngology，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022，China，Department，of Otorhinolaryngology，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022，China，Department，of Otorhinolaryngology，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022，China，Department，of Otorhinolaryngology，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022，China，Laboratory of Virology，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022，China |
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| 基金项目： | This project was supported by grants from National Excellent Young Scientists Foundation of China (No.39925035)
the Major Clinical Project of Ministry of Health (No.22012332). |
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| 摘 要： | The effect of vascular endothelial growth factor (VEGF) overexpression on matrix met-alloproteinase-2 (MMP-2) in nasopharyngeal carcinoma (NPC) cells in vitro and the possible mechanism involved were investigated, and the correlation between the expression of VEGF and MMP-2 in NPC evaluated. The NPC cells were transfected with PAd-trackVEGF165 plasmid. The expression levels of VEGF and MMP-2 mRNA and protein in NPC cells were detected by semi-quantitative RT-PCR and Western blot respectively. It was found that the expression of VEGF and MMP-2 mRNA and protein was significantly increased in NPC cells after transfection of VEGF165. It was concluded that the expression of VEGF was correlated to the in vitro invasion of NPC cells, and the induction of MMP-2 by VEGF was a key process of NPC cell invasion.
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Vascular Endothelial Growth Factorl65-regulated Nasopharyngeal Carcinoma Cell Lines Invasion and Migration Involve Expression and Activation of Matrix Metalloproteinase-2 |
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| WANG Yanjun,KONG Weijia,YUE Jianxin SUN Dawei,LI Wei YAOQi,SUN Yu,DONG Jihua.Vascular Endothelial Growth Factorl65-regulated Nasopharyngeal Carcinoma Cell Lines Invasion and Migration Involve Expression and Activation of Matrix Metalloproteinase-2[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2006(5). |
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| Authors: | WANG Yanjun KONG Weijia YUE Jianxin SUN Dawei LI Wei YAOQi SUN Yu DONG Jihua |
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| Affiliation: | WANG Yanjun,KONG Weijia,YUE Jianxin SUN Dawei,LI Wei YAOQi,SUN Yu,DONG Jihua Department of Otorhinolaryngology,Laboratory of Virology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China |
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| Abstract: | The effect of vascular endothelial growth factor (VEGF) overexpression on matrix met-alloproteinase-2 (MMP-2) in nasopharyngeal carcinoma (NPC) cells in vitro and the possible mechanism involved were investigated, and the correlation between the expression of VEGF and MMP-2 in NPC evaluated. The NPC cells were transfected with PAd-trackVEGF165 plasmid. The expression levels of VEGF and MMP-2 mRNA and protein in NPC cells were detected by semi-quantitative RT-PCR and Western blot respectively. It was found that the expression of VEGF and MMP-2 mRNA and protein was significantly increased in NPC cells after transfection of VEGF165. It was concluded that the expression of VEGF was correlated to the in vitro invasion of NPC cells, and the induction of MMP-2 by VEGF was a key process of NPC cell invasion. |
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| Keywords: | nasopharyngeal carcinoma cells vascular endothelial growth factor matrix metallo-proteinase-2 gene transfection |
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