喜树细胞悬浮培养中生理生化指标的测定

以喜树(Camptotheca acuminata Decsne.)幼嫩叶片为材料,诱导和筛选愈伤组织,进行细胞悬浮培养。初步研究了液体培养条件下光照对喜树细胞生长和生理生化特性的影响。结果显示：在光照条件下培养细胞生长周期30d,在黑暗条件下培养细胞生长周期为27d,细胞的增殖曲线呈“S”形;在两种不同光照条件下,其培养液pH值先下降后回升;培养细胞中可溶性蛋白质含量及过氧化物酶活性均出现两个峰值,但出现的时间不同。

Mensuration of Physiological and Biochemical Characters on the Cell Suspension Culture of Camptotheca acuminata Decsne.

With the young leaves of Camptotheca acuminata Decsne. callus are induced and screened, then establish the suspension cultures, the influences of light on the cell growth and physiological and biochemical characters are primarily studied. The results are as follows:When Camptotheca acuminata Decsne. is cultured in the light and in the dark, both of their cell growth curves are like "S", but their growth circles are about 30 days and 27 days respectively; The pH of cultured mediums goes down at first, then goes up gradually; soluble protein contents and peroxides activities of cultured cell have two peaks, but they were on the different days.