Molecular divergence of the soil yeasts <Emphasis Type="Italic">Williopsis</Emphasis> sensu stricto |
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Authors: | E?S?Naumova D?O?Gazdiev Email author" target="_blank">G?I?NaumovEmail author |
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Affiliation: | (1) State Research Institute of Genetics and Selection of Industrial Microorganisms, Pervyi Dorozhnyi proezd 1, 113545 Moscow, Russia;(2) Botanical Garden, Amur Scientific Center, Far East Division, Russian Academy of Sciences, Blagoveshchensk, 675000, Russia;(3) Amur Integrated Research Institute, Amur Scientific Center, Far Eastern Division, Russian Academy of Sciences, Relochnyi per. 1, Blagoveshchensk, 675000, Russia |
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Abstract: | Fifty-three strains of Saturn-spored yeasts were analyzed by means of restriction analysis of the amplified fragment of rDNA comprising the 5.8S rRNA gene and the internal transcribed spacers ITS1 and ITS2. The use of endonucleases HaeIII and MspI enabled clear differentiation of yeast species Williopsis mucosa, W. salicorniae, Zygowilliopsis californica, and Komagataea pratensis and the Williopsis sensu stricto complex. The minisatellite primer M13 was proposed for differentiation between sibling species of Williopsis sensu stricto, which have identical restriction profiles. PCR with primer M13 enabled reidentification of a number of collection strains, species identification of Saturn-spored isolates from the Far East, and detection of three strains affiliated to novel taxa. The latter have unique PCR profiles and differ in the nucleotide sequences of ITS1 and ITS2 fragments of rDNA. Possible variations in the results obtained with different molecular methods are discussed.Translated from Mikrobiologiya, Vol. 73, No. 6, 2004, pp. 768–776.Original Russian Text Copyright © 2004 by Naumova, Gazdiev, Naumov. |
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Keywords: | Williopsis sensu stricto Zygowilliopsis Komagataea RFLP analysis ITS1 and ITS2 rDNA |
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