| 实时荧光定量PCR技术快速检测麻疹病毒核酸的研究 |
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| 引用本文： | 卢亦愚,严菊英,冯燕,史雯,茅海燕.实时荧光定量PCR技术快速检测麻疹病毒核酸的研究[J].浙江预防医学,2004,16(11):1-2,7. |
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| 作者姓名： | 卢亦愚 严菊英 冯燕 史雯 茅海燕 |
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| 作者单位： | 浙江省疾病预防控制中心,浙江,杭州,310009 |
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| 摘 要： | 目的建立以特异性荧光探针为特点的TaqMan荧光定量RT-PCR方法,对麻疹病毒的核酸进行检测.方法对设计的引物与探针进行了筛选与条件优化,克服了常规RT-PCR定性检测的不足.结果具有对麻疹病毒检测的高度特异性与准确性,检测的敏感度可达0.1 TCID50,从病毒核酸提取至检测完成仅需3个多小时,操作简便,而且大大减少了常规PCR扩增产物的污染机会.结论采用该方法对麻疹病毒与相关的临床样本进行了检测,均获得了满意的结果,为麻疹病毒的分子生物学检测,提供了一种新方法.
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| 关 键 词： | 荧光定量聚核酶链反应 逆转录-聚合酶链反应(RT-PCR) TaqMan探针 麻疹病毒 检测 |
| 文章编号： | 1007-0931(2004)11-0001-03 |
| 修稿时间： | 2004年6月8日 |
TaqMan-based Real-time PCR Assay for Quick Detection of Measles Virus RNA |
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| LU Yi yu,YAN Ju ying,FENG Yan,et al..TaqMan-based Real-time PCR Assay for Quick Detection of Measles Virus RNA[J].Zhejiang Journal of Preventive Medicine,2004,16(11):1-2,7. |
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| Authors: | LU Yi yu YAN Ju ying FENG Yan |
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| Abstract: | Objective To establish a real time PCR assay based on Taq Man chemistry for detection of measles virus RNA. Methods The primers and probes were screened, and reactive condition was optimized. Results The specificity for detection of measles virus RNA was high, and the sensitivity was 0 1 TCID 50 . It only took three hours to complete the whole course, while the chance contamination decreased obviously compared to conventional PCR. Some clinic samples were detected by this method, and the results were satisfactory. Conclusions The TaqMen based real time PCR is rapid,sensitive and reliable for detection of measles virus. |
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| Keywords: | Real time PCR RT PCR TaqMan based probe Measles virus Detection |
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