纤维素酶-超声辅助提取玉米须槲皮素工艺优化

目的采用纤维素酶-超声法从玉米须中提取槲皮素,得到最佳工艺,为进一步开发玉米须资源提供依据。方法以槲皮素得率为指标,通过单因素试验,研究乙醇体积分数、液料比、超声时间、超声功率及酶用量对槲皮素得率的影响,利用正交法对影响槲皮素得率的上述5个因素进行优化。结果最佳提取工艺参数为乙醇体积分数50%、液料比25:1(m L/g)、超声时间40 min、超声功率200 W、酶用量0.014 mg。在此条件下,通过3次验证实验,测得槲皮素的得率为(0.374±0.023)%。结论采用正交法优化纤维素酶-超声法提取玉米须槲皮素的工艺具有可行性。

Optimization on extraction process of quercetin in corn silk by cellulase and ultrasound

Objective The cellulase-ultrasonic synergistic extraction conditions of quercetin from corn silk were optimized to improve the utilization of corn silk. Methods On the basis of single-factor tests, the extraction of quercetin was investigated with response to five variables, including ethanol concentration, liquid/solid ratio, ultrasonic time, ultrasonic power and enzyme dosage using orthogonal method. Results The optimal cellulase-ultrasonic synergistic extraction conditions for quercetin from corn silk were as follows: ethanol concentration was 50%, liquid/solid ratio was 25:1 (mL/g), ultrasonic time was 40 min, ultrasonic power was 200 W, and enzyme dosage was 0.014 mg. The yield of quercetin was (0.374±0.023)% under these conditions. Conclusion It is feasible to use orthogonal method to optimize the cellulase -ultrasonic synergistic extraction of quercetin in corn silk.