CXCR3在急性白血病患者外周血淋巴细胞的表达及其意义

目的探讨急性白血病(AL)患者外周血T淋巴细胞趋化因子受体CXCR3表达及其临床意义。方法采用流式细胞术结合直接免疫荧光法检测133例AL以及33例对照组外周血T淋巴细胞CXCR3表达。结果 AL患者外周血T淋巴细胞表面趋化因子受体CXCR3的表达阳性率明显高于对照组,分别为(28.24±18.14)%和(12.19±3.89)%(P<0.05)。在各型AL中,以B-ALL的表达率最高(40.14±17.56)%,而T-ALL型为(14.06±8.29)%,仅略高于正常对照(P>0.05);AL髓外浸润患者外周血T淋巴细胞CXCR3的表达为(34.54±19.87)%,明显高于非髓外浸润患者(20.44±12.15)%(P<0.01)。但AL患者外周血T淋巴细胞CXCR3的表达与白细胞总数、幼稚细胞百分率及CD3阳性率表达无关。结论 AL外周血T淋巴细胞CXCR3的表达可能与白血病的迁移、浸润密切相关。

The Expression and Significance of CXCR3 on T Lymphocytes from Peripheral Blood Cells in Patients with Acute Leukemia

Objective To study the expression and clinical significance of the chemokine receptor CXCR3 on T lymphocytes from peripheral blood cells in patients with acute leukemia.Methods Flow cytometry and direct immuofluorescence staining were used to detect the expressions of CXCR3 on T lymphocytes from peripheral blood cells in 133 cases of AL and 33 cases of normal controls.Results The positive expression rate of chemokine receptor CXCR3 on the surface of T lymphocyte from the peripheral blood cell in the patients with AL was(28.24±18.14)%,significantly higher than the control groups(12.19±3.89)%(P<0.05).Among different subtypes of AL,the expression rate in B-ALL was the highest(40.14±17.56)%,while T-ALL,with expression rate of(14.06±8.29)%,was only slightly higher than the normal control group.The CXCR3 expression on T lymphocytes in AL patients with extramedullary filtration was(34.54±19.87) %,and was significantly higher than those without extramedullary filtration(20.44±12.15) %(P<0.01).However,the expression of CXCR3 on the T lymphocytes from the peripheral blood cells in AL patients was not correlated to the WBC count,the percentage of blast cells,as well as CD3 positive rate.Conclusion The CXCR3 expression on T lymphocytes from the peripheral blood cells in patients with AL might be closely associated with migration of the leukemic cells and extramedullary organ infiltration.