A sensitive LC–MS/MS method to quantify loureirin B in rat plasma with application to preclinical pharmacokinetic studies |
| |
Authors: | Yujuan Li Yongzhi Li Zhezhe Yang Nian Xin Yulin Deng |
| |
Affiliation: | aSchool of Life Science and Technology, Beijing Institute of Technology, No. 5 Zhongguancun South Street, Beijing 100081, China;bChina Astronaut Research and Training Center, No. 28 Beiqing Street, Beijing 100094, China |
| |
Abstract: | A novel method for the quantification of loureirin B in rat plasma using high-performance liquid chromatography/tandem mass spectrometry (LC–MS/MS) was developed. Loureirin B and internal standard (buspirone) were extracted by liquid–liquid extraction and separated on a Agilent XDB C18 column (50 mm × 4.6 mm, 5 μm). As mobile phase a binary mixture of methanol (containing 0.1% formic acid)–water (containing 0.1% formic acid) was delivered by a Shimadzu LC-20AD pump in gradient mode at a flow rate of 0.4 ml/min in a run time of 5.0 min. The detector was a Q-trap™ mass spectrometer with an electrospray ionization (ESI) interface operating in the multiple reaction monitoring (MRM) mode. The calibration curve of loureirin B in plasma showed good linearity over the concentration range of 0.08–100 ng/ml. The limit of detection and limit of quantification were 0.03 ng/ml and 0.08 ng/ml, respectively. Intra- and inter-day precisions (as relative standard deviation) in all samples were both within 15%. The validated method was successfully applied to a preliminary pharmacokinetic study of loureirin B in rats. After oral administration of 16 g/kg longxuejie to rats, the main pharmacokinetic parameters tmax, Cmax, t1/2, Ke and AUC0–T were 0.8 h, 7.99 μg/l, 1.94 l h, 0.365/h, and 22.21 μg h/l, respectively. |
| |
Keywords: | Loureirin B Longxuejie Rat plasma Pharmacokinetics LC– MS/MS |
本文献已被 ScienceDirect 等数据库收录! |
|