日本血吸虫未成熟虫卵26/28kDa抗原诱导抗雌虫生殖和抗卵胚发育免疫的研究

目的 :探讨日本血吸虫未成熟虫卵的 2 6/ 2 8k Da抗原 ( SIEA2 6/ 2 8k Da)诱导小鼠产生抗雌虫生殖免疫的效果。方法 :采用纯化的 SIEA2 6/ 2 8k Da抗原以及 SIEA- I抗原 ,分别免疫BAL B/ c小鼠 ,于攻击感染后 4 6d进行粪卵、组织内虫卵定量。结果 :证明 SIEA2 6/ 2 8k Da抗原能诱导小鼠产生抑制雌虫生殖的免疫力。与对照组比较 ,SIEA2 6/ 2 8k Da抗原免疫鼠减虫虽不明显 ,但肝组织内总卵数、成熟卵数和粪卵数 ( EPG)分别减少 4 8.1%、83.6%、87.3% ,死亡卵数明显增加 ( P<0 .0 0 1)。此外 ,未纯化的 SIEA和纯化的 SIEA2 6/ 2 8k Da抗原免疫组均见雌虫子宫内虫卵数下降 ,分别达 4 0 .9%、54.8% ,而 SEA和 SIEA- I抗原免疫未见上述效应。结论 :提示抗雌虫生殖免疫和抗卵胚发育的效应 ,主要与 SIEA2 6/ 2 8k Da蛋白组分有关。

STUDIESON ANTI-FEMALE FECUNDITY AND ANTI- EMBRYONATION IMMUNITY INDUCED IN MICE WITH2 6/2 8k Da ANTIGENSOF IMMATURE EGGS OF SCHISTOSOMA JAPONICUM

AIM:To observe the effect of antiembryonation and anti- female fecundity immunity in mice induced by the 2 6 /2 8k Da antigens purified from soluble immature eggs antigen (SIEA) of Schistosoma japonicum.METHODS:BAL B/c mice were immunized with SIEA 2 6 /2 8k Da or SIEA- I antigen to examine if these antigens can induce anti- disease immunity as determined by egg burden in liver,intestines and faeces on day46 after challenge infection with cercariae of S.japonicum.RESUL TS:A comparison of the egg burden in tissues and faeces on day 46 after challenge infection showed that the number of mature eggs in the livers,intestines and faeces was significantly reduced in mice immunized with SIEA2 6 /2 8 k Da by83.6 % ,93.3% and87.3% ,respectively,whereas the number of dead eggs was much greater in the tissues of immunized mice than in that of control mice. In addition,the number of eggs in the uteri of female adults was obviously decreased in mice immunized with SIEA 2 6 /2 8k Da antigens or with the SIEA. However,these immune responses were not in- duced by the SEA or SIEA- I.CONCLUSION:The2 6 /2 8k Da antigens were major compo- nents in SIEA responsible for both anti- fecundity and antiembryonation immunity.