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| Bmi-1基因在脐血间充质干细胞增殖中的作用 | |
| 引用本文： | 顾建娟,韩素萍,李晓波.Bmi-1基因在脐血间充质干细胞增殖中的作用[J].临床儿科杂志,2009,27(7). |
| 作者姓名： | 顾建娟 韩素萍 李晓波 |
| 作者单位： | 1. 南京医科大学第一附属医院妇产科,江苏南京,210029;扬州大学临床医学院,江苏扬州,225001 2. 南京医科大学第一附属医院妇产科,江苏南京,210029 3. 扬州大学临床医学院,江苏扬州,225001 |
| 摘 要： | 目的 研究Bmi-1基因对脐血间充质干细胞(MSCs)增殖的影响.方法 培养人脐血MSCs,先利用RNA干扰技术抑制第3和6代MSCs Bmi-1基因的表达,用real-time PCR检测Bmi-1基因的表达,免疫组织化学检测BrdU掺入率来观察脐血MSCs增殖的能力;再通过连续传代至细胞增殖能力下降,利用real-time PCR检测Bmi-1基因在细胞增殖能力下降前后表达量的变化.结果 经过RNA干扰后,Bmi-1基因表达量显著减少;RNA干扰后的48 h、96 h、168 h,Bmi-1转录水平分别下降为正常的(28.67.±10.39)%、(24.46±12.15)%、 (32.68.±9.34)%.脐血MSCs的BrdU掺入率在RNA干扰前为(38.97 ±5.02)%,干扰后显著下降为(12.62 ±2.91)%.此外,当脐血MSCs体外连续传至12～15代时,伴随其增殖能力的下降,表现在Brdu掺入率降至(4.18 ±1.53)%,Bmi-1转录水平也显著下降(P<0.01).结论 RNA干扰抑制脐血MSCs Bmi-1基因表达后,细胞增殖能力明显下降;细胞连续体外传代至增殖能力下降后,Bmi-1基因表达显著下降.说明Bmi-1基因在脐血MSCs的增殖过程中发挥重要作用. |
| 关 键 词： | 脐血 间充质干细胞 细胞增殖 RNA干扰 |
Role of Bmi-1 in the proliferation of mesenchymal stem cells derived from umbilical cord blood |
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| GU Jian-juan,HAN Su-ping,LI Xiao-bo.Role of Bmi-1 in the proliferation of mesenchymal stem cells derived from umbilical cord blood[J].The Journal of Clinical Pediatrics,2009,27(7). | |
| Authors: | GU Jian-juan HAN Su-ping LI Xiao-bo |
| Affiliation: | 1.Department of Obstetrics and Gynecology;The First Affiliated Hospital of Nanjing Medical University;Nanjing 210029;Jiangsu China;2.Medical College of Yangzhou University;Yangzhou 225001;Jiangsu;China |
| Abstract: | Objective To determine the effect of Bmi-1 on the proliferation of mesenchymal stem cells(MSCs) derived from umbilical cord blood.Methods MSCs were isolated from umbilical cord blood and cultured in vitro.The RNA interference technology was used to inhibit the expression of MSCs Bmi-1 gene in passage 3 and passage 6.The expression of Bmi-1 gene was measured by quantitative real-time PCR,and the proliferation of MSCs was measured by Brdu incorporation which was detected by immunohistochemistry.After serial p... |
| Keywords: | Bmi-1 |
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