| 用两株McAb及BA—ELISA法检测肺癌患者血清中肺癌相关抗原 |
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| 引用本文： | 薛伟,侯健存.用两株McAb及BA—ELISA法检测肺癌患者血清中肺癌相关抗原[J].中国医学科学院学报,1991,13(1):63-67. |
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| 作者姓名： | 薛伟 侯健存 |
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| 作者单位： | 中国医学科学院基础医学研究所，中国医学科学院基础医学研究所 北京 硕士研究生，北京 |
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| 摘 要： | 肿瘤患者血清中肿瘤相关抗原含量甚微,通常需要高度敏感的测定系统方能进行检测.生物素-亲和素系统(BSA)是近年来建立的一种生物反应放大系统,由于BSA亲合力高、特异性强反应迅速,故自70年代后期引入免疫技术以来,大大改善和丰富了免疫学试验,成为新的检测系统.本实验用生物素标记两株抗人肺癌McAb——Lc86aE4和Lc86aC5,建立了BA-ELISA方法,并对肺癌患者血清中肺癌相关抗原(lung carcinoma associated antigen,LAA)进行了检测,证实BA-ELISA法具有特异、敏感及重复性好等优点.
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| 关 键 词： | 肺肿瘤 血清 相关抗原 检测 McAb |
Using two monoclonal antibodies to detect lung carcinoma associated antigen in the sera of patients with lung cancer by BA-ELISA] |
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| W Xue,J Hou.Using two monoclonal antibodies to detect lung carcinoma associated antigen in the sera of patients with lung cancer by BA-ELISA][J].Acta Academiae Medicinae Sinicae,1991,13(1):63-67. |
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| Authors: | W Xue J Hou |
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| Affiliation: | Institute of Basic Medical Sciences, Beijing. |
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| Abstract: | In this study two McAbs (LC86aE4 and LC86aC5) were chosen to detect circulating lung carcinoma associated antigen (LAA) in serum samples from 58 patients with lung cancer, 29 patients with nonmalignant thoracic diseases and 30 healthy donors by biotin-avidin (BA)-ELISA. Our data indicate that BA-ELISA was specific, sensitive, reproducible and simple. The rates of positive detection of LAAE4 or LAAC5 alone were found to be higher (44.8% and 48.3% respectively) in patients with lung cancer than in those with nonmalignant thoracic diseases (13.7%) and in normal donors (6.7%) (P less than 0.01). Furthermore, the combined detection of LAAE4 and LAAC5 resulted in a higher positive rate (62.3%) in lung cancer patients. The clinical application of these McAbs and the assay in the diagnosis of lung cancer is discussed. |
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