新加生脉饮对阿霉素所致心肌细胞凋亡及Bax、Bcl-2、Caspase-3蛋白表达的影响

目的通过观察新加生脉饮对阿霉素所致大鼠心肌细胞凋亡的逆转和防治作用,为阿霉素心肌损伤的临床治疗奠定实验基础。方法利用阿霉素的心脏毒性诱导心肌细胞凋亡大鼠模型。将40只雄性SD大鼠随机分成4组:对照组、阿霉素模型组、卡托普利(10 mg/kg)组、新加生脉饮(3.75 g/kg)组,ig给予相应药物,每日1次,共给药6周。实验过程中观察大鼠的精神、活动、进食、毛色等情况,治疗6周后测定心功能、大鼠左室肥厚指标,HE染色观察心肌病理变化,TUNEL染色观察心肌细胞凋亡情况,免疫组化法检测心肌Caspase-3、Bcl-2、Bax蛋白表达情况。结果与对照组比较,模型组大鼠心功能显著降低。与模型组比较,各药物治疗后可不同程度改善大鼠心功能情况,其中新加生脉饮组疗效显著。与对照组比较,模型组大鼠心体比、左室肥厚指数、心肌细胞凋亡指数均明显升高。与模型组比较,卡托普利及新加生脉饮可不同程度地改善大鼠心肌肥厚及心肌细胞凋亡情况。与对照组比较,模型组大鼠心肌Bcl-2蛋白表达水平降低,Bax、Caspase-3蛋白表达水平升高;与模型组比较,卡托普利及新加生脉饮可不同程度地增加大鼠心肌Bcl-2蛋白表达水平,减低Bax、Caspase-3蛋白表达水平。结论新加生脉饮能改善心肌细胞凋亡大鼠心功能情况,通过降低心肌细胞中Caspase-3蛋白的表达或抑制其活性,增加Bcl-2蛋白的表达,降低Bax蛋白的表达,上调Bcl-2/Bax,从而抑制心肌细胞凋亡。

Effect of New Shengmai Decoction on apoptosis and protein expression of Bax, Bcl-2, and Caspase-3 of cardiomyocyte induced by adriamycin

Objective To observe the reversal and prevention effect of New Shengmai Decoction on the rats'' cardiomyocyte apoptosis induced by adriamycin, and provide the experimental foundation basis for the clinical treatment of myocardial injury induced by adriamycin. Method The rat models with cardiomyocyte apoptosis were established by adriamycin. Forty male SD rats were divided randomly into four groups, including the normal group, the adriamycin model group, the captopril group and the New Shengmai Decoction group. During the experiment, the mental state, activity, feeding, hair color and other conditions of the rats were observed. After 6 weeks of treatment, the cardiac function and left ventricular hypertrophy of rats were measured and the pathological changes of myocardium were observed by HE staining. And the apoptosis of myocardial cells was observed by TUNEL staining and protein expressions of Caspase-3, Bcl-2, and Bax were detected by immunohistochemistry. Results Compared with the control group, the cardiac function of the model group was significantly decreased. Compared with the model group, the cardiac function of rats after the different drugs'' treatment could be improved in the different degrees and the effect of the New Shengmai Decoction group was significant. Compared with the control group, the heart body ratio, left ventricular hypertrophy index and myocardial cell apoptosis index in the model group were all significantly increased. Compared with the model group, the captopril group and the New Shengmai Decoction group ameliorated cardiac hypertrophy and myocardial cell apoptosis in rats. Compared with the control group, the expression level of bcl-2 protein in the model group was decreased, while the expression level of Bax and Caspase-3 protein was increased. Compared with the model group, captopril and the New Shengmai Decoction increased the expression level of bcl-2 protein and decreased the expression level of Bax and Caspase-3 protein.Conclusion The New Shengmai Decoction can improve the cardiac function and lessen cardiomyocyte apoptosis of rats. It can also decrease the expression of protein Caspase-3 in the cardiac muscle of rats or inhibit its activity. In order to restrain cardiomyocyte apoptosis, the New Shengmai Decoction can increase the expression of protein Bcl-2 and decrease the expression of protein Bax through improving the expression of Bcl-2/Bax in the cardiac muscle of rats.