塞来昔布对人肝癌细胞增殖抑制及放疗增敏作用

目的探讨塞来昔布对肝癌细胞huh-7增殖抑制及放疗增敏作用。方法以人肝癌细胞株huh-7为研究对象,以塞来昔布和高能射线作为干预手段,采用四唑氮蓝还原法(MTT),检测不同浓度塞来昔布和作用不同时间对huh-7细胞的增殖抑制作用,采用流式细胞技术,检测塞来昔布联合放疗对huh-7细胞凋亡和周期的影响。结果 MTT显示塞来昔布对人肝癌细胞huh-7生长有显著抑制作用,且呈时间和剂量依赖性;塞来昔布对肝癌放疗具有明显增敏作用。与对照组比较,药物组G0/G1期细胞比例增加,S期细胞比例减少,G2/M变化无明显意义;照射组G2/M期细胞比例升高,联合组G0/G1期细胞比例增加,S期细胞比例减少。结论塞来昔布能提高人肝癌细胞株huh-7放疗敏感性作用,其作用可能是通过诱导细胞凋亡、改变细胞周期时相分布来实现的。

Celecoxib on Human Hepatoma Cell Proliferation Inhibition and Radiosensitization

Objective To investigate the function of celecoxib on human hepatocellular carcinoma cell huh-7 proliferation inhibition and radiotherapy sensitization.Methods In human liver carcinoma cell line huh-7 as the research object,using celecoxib and high-energy ray as a means of intervention,the function of celecoxib on cell proliferation inhibition was observed in different concentration and action time by MTT,and the apoptosis and cell cycle effect of celecoxib combined with radiotherapy on hepatoma cells were evaluated by using flow cytometric technique.Results ComPared with control group,the absorption value decreased significantly with the increase of celecoxib concentration and exposure time.Celecoxib on liver cancer radiotherapy sensitization effect is obvious.Compared with the control group,drug group increased the proportion of G0/G1 phase cells,reduced the proportion of S phase cells,G2/M had no obvious meaning;G2/M phase cells increased in the radiotherapy group;the combination group increased the proportion of G0/G1 phase cells,S phase cells reduced,Cell proliferation was inhibited.Conclusion Celecoxib can improve the human hepatoma cell line huh-7 sensitivity to the role of radiotherapy,its role may be through the induction of apoptosis,cell cycle phase distribution changes to achieve.