高效液相色谱分离氨基酸和胺的非对映异构体

本文报道了使用三种不同类型十八烷基键合填充荆分离氨基酸及胺的非对映异构体,实验结果表明,我所自制的微粒型GYT-C₁₈填充剂分离效果较好,所试验的七种化合物中有六种达到基线分离。并发现化合物的构型同出峰的次序之间无规律性关系。

SEPARATION OF AMINO ACIDS AND AMINES AS DIASTEREOISOMERS BY HIGH PERFORMANCE LIQUID CHROMATOGRPHY

Three kinds of chemically bonded micro-porous silica (10 um partisil ODS-2, 10 μm GYT-C18 and 5 μm GYT-C₁₈) were used for separation of amino acids and amines diastereoisomers, 5μm and 10 μm GYT-C₁₈, prepared in our Institute, with good efficiency. The theoretical plates of Partisil ODS, 10μm and 5μm GYT-C₁₈ are 10000plates/m, 46000plates/m and 53000plates/m, respectively. Five racemic amino acids were separated as the diastereoisomeric mixture of D-camphor-β-suphonyl-p-nitrobenzyl ester and two racemic aralkylamines as N-D-camphor-β-sulphonylamines. The column slurry-packed with 10 μm GYT-C₁₈ gave the most favorable results. For seven racemates tested, six pairs of diastereoisomers gave baseline separation.The experimental data showed that no conclusive correlation between elution order and absolute configurations could be determined.