西洋参总皂苷的分离纯化工艺

目的:研究大孔吸附树脂法纯化西洋参总皂苷的工艺条件及参数.方法:以西洋参总皂苷含量为指标,考察上样液质量浓度、洗脱溶媒及其用量、除杂溶媒及其用量等条件,优选大孔树脂纯化工艺条件.结果:西洋参总皂苷最佳纯化工艺为0.8 BV上样液(0.5 g·mL-1)通过HPD-300大孔树脂,4 BV水洗,3 BV 10％乙醇洗脱,5 BV 70％乙醇洗脱,收集70％乙醇洗脱液,以人参皂苷Rg1、人参皂苷Rb1和人参皂苷Re计总皂苷纯度为50.83％.结论:采用该优选方法可较好地分离、纯化西洋参总皂苷.

Separation and Purification Technology of Total Saponins from Panax quinquefolium by Macroporous Resin

Objective: To optimize purification technology parameters of total saponins from Panax quinquefolium by macroporous resin. Method: With the content of total saponins as index,purification technology was optimized by investigating the concentration of sample liquid,elution solvent and the amount of it,impurity solvent and the amount of it,et al. Result: Optimum purification technology was:0.8 BV sample liquid(0.5 g·mL^-1) passed HPD-300 resin,eluted by 4 BV water and 3 BV 10% ethanol,eluted by 5 BV 70% ethanol,collected eluate,purity of total saponins was up to 50.83%(sum of ginseng saponin Rg₁,ginseng saponin Rb₁ and ginseng saponin Re). Conclusion: This method had a better separation and purification capacity for total saponins for Panax quinquefolium.