平顶山地区普通人群与幽门杆菌患者免疫型的对比分析

目的]建立幽门螺杆菌(Hp)免疫型分析方法,探讨不同人群Hp免疫性的临床意义。方法]将健康体检者200例作为普通人群组,将有上消化道症状的门诊患者325例作为门诊患者组,进行病史调查并抽血作Hp-IgG免疫印迹法检测。比较两组之间Hp-IgG的检出率及Hp毒力相关特异印迹带的检出率及反应强度的差异。结果]门诊患者组与普通人群组近3个月受检者上消化道症状的发生率分别为100%和33.5%,用药史为76.3%和26.5%。但两组Hp-IgG的阳性率分别为69.8%和65.0%,CagA、VacA、UreA以及低分子量蛋白的抗体检出率分别为58.5%、55.7%、49.2%、52.6%和52.0%、50.5%、48.0%、46.55%,两组间差异无统计学意义。而CagA、VacA、UreA以及低分子量蛋白的抗体滴度强弱及比例(免疫型)有明显的差异。结论]研究结果表明,Hp感染并不是上消化道疾病出现症状的最主要原因,而与Hp感染的免疫型有明显的关系。Hp的致病作用与Hp长期感染及毒素的慢性作用有关。Hp感染的免疫型可作为评价Hp致病性及指导临床根除Hp的重要作用依据。

THE ASSOCIATION AND MECHANISM ON IMMUNE TYPES OF HELICOBACTER PYLORI PATIENTS AND OTHERS IN PINGDINGSHAN REGION

Objective] To establish a analytic method for immune types of Helicobacter pylori (H.py-loyi) and to confirm the clinical significance of the immune types. Methods] Three hundred and twenty-five patients with different digestive symptoms (Group A) and 200 health people (Gyoup B) were enrolled the study. Medical history survey and serum test for H. pylori-lgG by Western blotting were performed. The difference of H.pylori Western Blotting results was compared between the two groups. Results] The incidence ...