| 马铃薯延薯4号再生体系建立 |
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| 引用本文： | 康哲秀,吴京姬,郎贤波,许震宇,玄春吉,南哲佑.马铃薯延薯4号再生体系建立[J].黑龙江农业科学,2016(12). |
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| 作者姓名： | 康哲秀 吴京姬 郎贤波 许震宇 玄春吉 南哲佑 |
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| 作者单位： | 延边朝鲜族自治州农业科学院,吉林龙井,133400 |
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| 基金项目： | 国家马铃薯产业技术体系建设专项资金资助项目(CARS-10) |
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| 摘 要： | 为了建立马铃薯高效、稳定离体再生体系,以延薯4号无菌试管苗茎段为材料,通过对茎段再生体系的筛选,优化了培养基内最佳激素配比浓度。结果表明:马铃薯延薯4号无菌试管苗茎段诱导愈伤组织最适培养基为:MS+6-BA3.0 mg·L~(-1)+NAA1.0 mg·L~(-1)。茎段丛生芽分化的最适培养基为:MS+6-BA2.0mg·L~(-1)+NAA0.20mg·L~(-1)+G A_31.0mg·L~(-1)。
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| 关 键 词： | 马铃薯 延薯4号 茎段 愈伤组织诱导 植株再生 |
Establishment of the Regeneration System of Potato Variety Yanshu 4 |
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| Abstract: | To establish the highly efficient and stable in vitro regeneration system of potato ,the stem regenera‐tion system of the aseptic Yanshu 4 seedlings was screened and the hormone proportion of culture medium was optimized .The results showed that the optimum culture medium for the callus induction of explants from stem segments of Yanshu 4 was MS+6‐BA3 .0 mg·L‐1 +NAA1 .0 mg·L‐1 and the best culture medium for differen‐tiation of multiple buds inducted by stem segment callus was MS + 6‐BA2 .0 mg·L‐1 + NAA 0 .20 mg·L‐1 +GA3 1 .0 mg·L‐1 . |
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| Keywords: | potato Yanshu 4 stem segment callus induction plant regeneration |
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