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玄参炮制前后多糖含量的变化研究
引用本文:方学敏,曹岗,蔡银燕.玄参炮制前后多糖含量的变化研究[J].中华中医药学刊,2012(5):986-987.
作者姓名:方学敏  曹岗  蔡银燕
作者单位:杭州市推拿医院;浙江中医药大学;杭州市第三人民医院
基金项目:教育部国家一级学科中药学开放课题资助项目(2011ZYX2-006);浙江省中医药重点研究计划资助项目(2008ZA002)
摘    要:目的:测定并考察玄参炮制前后多糖的含量变化,以探讨玄参的炮制机理。方法:采用苯酚-硫酸法测定玄参炮制前后多糖的含量。结果:生品总糖含量为64.89%,得率为9.74%;制品总糖含量为65.90%,得率为7.89%,较生品多糖得率下降了19.02%。结论:玄参经蒸制后,多糖得率降低。实验结果为探讨玄参炮制机理提供了一定的依据。

关 键 词:玄参  多糖  苯酚-硫酸法  炮制

Studies on the Change of the Content of Polysaccharides in Crude and Processed Radix Scrophulariae
FANG Xue-min,CAO Gang,CAI Yin-yan.Studies on the Change of the Content of Polysaccharides in Crude and Processed Radix Scrophulariae[J].Chinese Archives of Traditional Chinese Medicine,2012(5):986-987.
Authors:FANG Xue-min  CAO Gang  CAI Yin-yan
Affiliation:1.Hangzhou Massage Hospital,Hangzhou 310009,Zhejiang,China;2.Zhejiang Chinese Medical University, Hangzhou 310053,Zhejiang,China;3.Hangzhou No.3 Peoples Hospital,Hangzhou 310009,Zhejiang,China)
Abstract:Objective:To determine the content of Polysaccharides in crude and processed Radix Scrophulariae,thus to approach the process mechanism of Radix Scrophulariae.Methods:Phenol-sulfuric acid method was used to determine polysaccharides in crude and processed Radix Scrophulariae.Results:After processing,the content of polysaccharides of Radix Scrophulariae decreased from 9.74%(content of total polysaccharidesbeing 64.89%) to 7.89%(content of total polysaccharidesbeing 65.90%),decreased by 19.02% as compared with that in crude Radix Scrophulariae.Conclusion:After processing,the content of polysaccharides decreases markedly.The results provide certain evidence for approaching the process mechanism of Radix Scrophulariae.
Keywords:Radix Scrophulariae  Processed  Polysaccharides  Phenol-sulfuric acid method
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