硫代乙酰胺诱导的肝硬化大鼠生长激素/胰岛素样生长因子-1轴的变化

目的探讨硫代乙酰胺诱导的肝硬化大鼠GH/IGF-1轴的变化.方法设正常组大鼠6只、肝硬化组大鼠6只,测大鼠肝功能、血糖和血清GH、IGF-l、IGFBP-3水平,用RT-PCR法检测肝ALBmRNA、IGF-l mRNA、IGFBP-3 mRNA表达.肝组织行HE染色和Ki67免疫组化染色.结果肝硬化组大鼠血清AST、ALT、ALP明显高于正常组,血清TBIL、ALB、血糖两组间无明显差别,血清GH水平明显高于正常组,而血清ICF-1、IGFBP-3水平明显低于正常组,肝ALB mRNA、IGF-1mRNA、IG阳P-3 mRNA表达水平明显低于正常组.肝硬化组大鼠镜下见假小叶形成,肝Ki67指数明显高于正常组.结论肝硬化大鼠存在GH/IGF-1轴的变化.

Changes in growth hormone/insulin like growth factor-1 axis of rats with thioacetamide-induced liver cirrhosis

Objective:To explore the changes in growth hormone (GH)/insulin like growth factor-1 (IGF-1) axis of rats with thioacetamide-induced liver cirrhosis. Methods:Six rats with thioacetamide-induced liver cirrhosis served as cirrhotic group and six normal rats as normal group. Liver function,blood glucose, serum GH, IGF-1 and IGF binding protein-3(IGFBP-3) were detected. Albumin (ALB) mRNA, IGF-1 mRNA and IGFBP-3 mRNA levels in liver were detected by RT-PCR. Liver pathological observation included HE staining and Ki67 immunohistochemistry staining.Results:In the cirrhotic group, serum AST, ALT, ALP GH levels were significantly higher but serum IGF-1 and IGFBP-3 levels were significantly lower and the expressions of hepatic ALB mRNA, IGF-1 mRNA and IGFBP-3 mRNA were significantly lower than those in normal group. Liver Ki67 labeling index was significantly higher in cirrhotic group.Conclusions:There is an obvious change in GH/IGF-1 axis of rats with thioacetamide-induced liver cirrhosis. Dectecting the change of GH/IGF-1 was helpful to choose nutrients.