林蛙卵油脂肪酸组分的分析研究

林蛙卵经石油醚和超临界CO2流体萃取得到林蛙卵油。把林蛙卵油甲酯化,用石英毛细管色谱/质谱法测定其脂肪酸组分。采用石油醚方法萃取得到的林蛙卵油分离出19种组分,不饱和脂肪酸含量为72.38%;采用超临界CO2流体萃取得到的林蛙卵油分离出26种组分,不饱和脂肪酸含量为69.65%。研究结果表明:两种方式获得的林蛙卵油脂肪酸主要组分及含量基本一致,均由C12-22脂肪酸组成,主要不饱和脂肪酸是十六碳烯酸、亚油酸、油酸、EPA(二十碳五烯酸)、DHA(二十二碳六烯酸)等。

The Analysis and Research of Components of Fatty Acid in the Ovum Oil of Rana chensinensis

The ovum oil of Rana chensinensis was obtained by the methods of supercritical CO2 fluid and petroleum etherextraction, and was converted to methyl easter. The components of its fatty acid were determined by quartz capillary GC/MS.19 kinds of fatty acids were separated from the oil obtained by the methods of petroleum ether extraction and 72.38% of themare unsaturated fatty acids. 26 kinds of fatty acids were separated from the oil obtained by the methods of supercritical CO2 fluidextraction and 69.65% of them are unsaturated fatty acids. The study result indicates that the main components and content ofthe fatty acid in the oil obtained by the two kinds of methods are basically same, and the main unsaturated fatty acids arehexadecenoic acid, linoleic acid, oleic acid, eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA).