不同浓度细胞种植脱细胞血管基质上构建组织工程血管

摘要 背景：前期研究表明制备的脱细胞血管基质适合平滑肌细胞和内皮祖细胞生长，但是共培养模型中细胞接种密度对血管基质上细胞覆盖率的影响尚不清楚。 目的：观察不同浓度度细胞种植在脱细胞血管基质上的生长情况。 方法：采用两步法进行细胞种植，先将不同细胞浓度血管平滑肌细胞种植在脱细胞血管基质上，培养3 d后再将不同浓度内皮祖细胞接种在平滑肌细胞-血管基质复合体上，构建片状组织工程材料，分别在内皮祖细胞种植后3 d、1周时间段获取标本，扫描电镜观察细胞在材料上的生长情况。 结果与结论：血管平滑肌细胞和内皮祖细胞在脱细胞基质表面生长良好。不同浓度细胞在基质上的排布不同；提高接种的浓度有利于在材料表面快速形成致密的细胞层。提示采用两步法以合适的浓度种植细胞于脱细胞基质上，可以构建组织工程血管。 关键词：内皮祖细胞；平滑肌细胞；组织工程血管；脱细胞血管基质；种植 doi:10.3969/j.issn.1673-8225.2010.47.010

Tissue engineered blood vessels constructed by implanting different densities of cells on decellularized vascular matrix

Abstract BACKGROUND: Previous studies have shown that decellularized vascular matrix prepared is suitable for the growth of smooth muscle cells and endothelial progenitor cells, but the effect of cell inoculation density on cell coverage of vascular matrix in co-culture model remains unclear. OBJECTIVE: To observe the growth of seeding cells at different densities implanted on decellularized vascular matrix. METHODS: Cell implantation was performed using two-step seeding method. Firstly, smooth muscle cells were seeded onto the decellularized vascular matrix with different densities, and cultured for 3 days, and then endothelial progenitor cells were then seeded onto the smooth muscle cells-vascular matrix compound with different densities, to construct lamellar tissue engineered materials. The samples were harvested at 3 days and 1 week after the implantation of endothelial progenitor cells, cell growth was observed by scanning electron microscope. RESULTS AND CONCLUSION: Smooth muscle cells and endothelial progenitor cells grew well on the decellularized vascular matrix, cells arranged differently on the matrix with different seeding densities; with the increase of seeding density, cells showed higher covering rate on the material surface. Tissue engineering blood vessels can be constructed by implanting suitable density of seeding cells to the decellularized vascular matrix using a two-step method.