中药半枝莲黄酮类成分的分离与结构鉴定

目的对中药半枝莲(Scutellaria barbata D.Don)全草的化学成分进行研究。方法应用大孔树脂、正相硅胶、反相ODS、Sephadex LH-20以及制备型高效液相等色谱法对中药半枝莲的化学成分进行了分离;并通过理化性质和光谱数据对化合物的结构进行了鉴定。结果分离鉴定了7个黄酮类化合物,分别为芹菜素(apigenin1,)、木犀草素(luteolin,2)、5,7,4'-三羟基-8-甲氧基黄酮(5,7,4'-trihydroxy-8-methoxyflvone,3)、芹菜素-7-O-β-D-葡萄糖醛酸苷(apigenin-7-O-β-D-glucuronide,4)、芹菜素-7-O-β-D-葡萄糖醛酸甲酯(apigenin-7-O-β-D-glucuronide methyl ester,5)、芹菜素-7-O-β-D-葡萄糖苷(apigenin-5-O-β-D-glucopyranoside,6)、山奈酚-3-O-β-D-芦丁糖苷(kaempferol-3-O-β-D-rutino-side,7)。结论化合物4-7为半枝莲植物中首次分离得到。

Isolation and identification of flavonoids of whole plant of Scutellaria barbata D.Don

Objective To study the chemical constituents of the whole plant of Scutellaria barbata D.Don. Methods The compounds were isolated by chromatographic methods including resin,silica gel,ODS,Sephadex LH-20 and preparation HPLC et al.,and identified by the chemical and physical methods,especially spectral analysis. Results Seven flavonoids were isolated.The structures of them were identified as apigenin( 1),luteolin( 2),5,7,4′-trihydroxy-8-methoxyflvone( 3),apigenin-7-O-β-D-glucuronide( 4),apigenin-7-O-β-D-glucuronide methyl ester(5),apigenin-5-O-β-D-glucopyranoside( 6)and kaempferol-3-O-β-D-rutinoside( 7),respectively. Conclusions Compounds 47 are obtained from S.barbata for the first time.