| 倒卵叶五加多糖提取分离工艺的优化 |
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| 引用本文： | 齐香君,陈立,朱爱华,曹爱兰.倒卵叶五加多糖提取分离工艺的优化[J].西北轻工业学院学报,2009,27(1):1-5. |
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| 作者姓名： | 齐香君 陈立 朱爱华 曹爱兰 |
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| 作者单位： | 齐香君,陈立,QI Xiang-jun,CHEN Li(陕西科技大学生命科学与工程学院,陕西,西安,710021);朱爱华,曹爱兰,ZHU Ai-hua,CAO Ai-lan(陕西省中药研究所,陕西,咸阳,712000)
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| 摘 要： | 通过正交试验优化了倒卵叶五加多糖的提取分离工艺．采用水提醇沉法提取多糖，通过单因素试验和L9（3^4）正交试验对影响多糖提取分离工艺的各因素进行了研究．所优化的倒卵叶五加多糖最佳提取分离工艺为用10倍量水提取3次，每次1h；醇沉分离工艺最佳条件为醇沉时乙醇体积分数859／6，料液比1：2（g／mL），静置14h．实验证明，该提取分离工艺稳定可靠．
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| 关 键 词： | 倒卵叶五加 单因素试验 正交试验 提取分离 多糖 |
STUDY ON EXTRACTION AND ISOLATION TECHNOLOGY OF POLYSACCHARIDE FROM ACANTHOPANAX OBOVATUS HOO |
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| QI Xiang-jun,CHEN Li,ZHU Ai-hua,CAO Ai-lan.STUDY ON EXTRACTION AND ISOLATION TECHNOLOGY OF POLYSACCHARIDE FROM ACANTHOPANAX OBOVATUS HOO[J].Journal of Northwest University of Light Industry,2009,27(1):1-5. |
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| Authors: | QI Xiang-jun CHEN Li ZHU Ai-hua CAO Ai-lan |
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| Affiliation: | QI Xiang-jun, CHEN Li, ZHU Ai-hua, CAO Ai-lan ( 1.School of Life Sciencc and Engineering, Shaanxi University of Science ＆ Technology, Xi＇an 710021, China;2.Shaanxi Institute of Traditional Chinese Medicine, Xianyang 712000, China) |
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| Abstract: | This paper investigated the extraction and isolation technology of polysaccharides from Acanthopanax obovatus Hoo. The single-factor experiments and orthogonal experi ments were adopted to analyze the effect of each factor in solid-liquid ratio, ethanol and ex traction time on the extraction percentage of the polysaccharides. The Results were indicated that ethanol and cxtraction lime significantly affected the extraction percentage of the poly- saccharides. The optimal was 10 limes water extracted 3 times, each 1 h. 1 ： 2 （g/m solid-liquid ratio. 85% ethanol precipitated. 14 h deposited. The extraction and iso technology of polysaccharides from Acanthopanax obovatus Hoo was simple, stable and efficient. |
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| Keywords: | Acanthopanax obovatus Hoo single-factor experiments orthogonal experiments extraction and isolation technology polysaccharide |
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