丙泊酚对大鼠缺血再灌注肾NF-κB、TNF-α表达的影响

目的 研究丙泊酚对大鼠肢体缺血再灌注损伤后肾组织中肿瘤坏死因子-α（tumornecrosisfactor-α，TNF-α）、核因子-κB（nuclear factor-κxB，NF-κB）表达的影响。方法 24只SD雄性大鼠，随机分为假手术对照组（A组），肢体缺血再灌注组（B组）和丙泊酚干预组（C组），每组8只。采用免疫组织化学方法检测TNF-α、NF-κB的表达，行图像分析半定量。结果 B组大鼠肾组织中TNF-α、NF-κB的表达明显高于A组（P〈0．05），C组明显低于B组（P〈0．05）。结论 肢体缺血再灌注后有明显肾损伤，丙泊酚对肢体缺血再灌注肾损伤有一定程度的保护作用，其机制可能与下调大鼠肢体缺血再灌注损伤肾组织中TNF-α、NF-κB的过度表达有关。

Effects of Propofol on Renal Expression of TNF-α and NF-κ B Following Hindlimb Ischemia/Reperfusion in Rats

Objective To investigate the effects of propofol on renal expression of tumor necrosis factor-α（TNF-α）and nuclear factor-κB（NF-κB）following hindlimb ischemia/reperfusion in rats. Methods Twenty-four SD rats were randomly allocated into three groups with 8 rats each ： control group （A）, ischemia/reperfusion group （B） and propofol group（C）. Expression of TNF-α and NF-κB in renal tissue were detected by immunohistochemical and semi- quantitated by image analysis. Results The expression of TNF-α and NF-κB were significantly higher in group B than those in group A ,but it＇s lower in group C than those in group B. Conclusion Propofol has protective effects on the kidney against injury induced by hindlimb ischemia/reperfusion,which may be related to a decrease in over expression of TNF-α and NF-κB in renal tissue of hindlimb ischemia/reperfusion injury in rats.