SLE与γ干扰素的基因型及血清水平的相关性

目的观察γ干扰素(IFN-γ)基因多态性各等位基因及基因型在系统性红斑狼疮(SLE)中的分布频率,初步分析其基因型及血清水平与SLE的相关性。方法采用序列特异性引物-聚合酶链反应(PCR-SSP)技术检测120例SLE患者和150名正常对照者IFN-γ的基因+874 A/T多态性,同时采用酶联免疫吸附试验(ELISA)检测血清IFN-γ水平。结果SLE组血清IFN-γ水平与对照组比较明显升高,且差异有显著性意义(P<0.05),IFN-γ基因+874 A/T多态性在两组人群中的分布差异有显著性意义(P<0.05),T等位基因携带者患SLE的风险是A等位基因的1.737倍(OR= 1.737,95%CI:1.193～2.529),携带T等位基因的SLE患者血清IFN-γ水平与不携带者比较明显升高,且差异有显著性意义(P<0.05)。结论IFN-γ基因+874 A/T多态性与SLE的发病具有相关性,T等位基因可能是SLE发病的遗传易感基因;携带T等位基因的个体可能通过促进IFN-γ的高度表达增加了SLE的发病风险。

The Serum Level and Genotype of Interferon-gamma in Patients with Systematic Lupus Erythematosus

Objective To study the relationship the allele frequencies and genotype distribution of Interferon-gamma(IFN-γ) gene polymorphism in patients with systematic lupus erythematosus(SLE),And to analyze association the serum levels and genotype of IFN-γwith SLE.Methods IFN-γgene + 874 A/T polymorphism was analyzed by the sequence specific primers-polymerase chain reaction(PCR-SSP) methods in 120 patients with SLE and 150 healthy controls,and the serum level of IFN-γwas determined by enzyme-linked immunosorbent assay(ELISA).Results The SLE group showed significantly higher serum levels of IFN-γthan that in control group(P<0.05),The distributions of IFN-γgene + 874 A/T polymorphism was significantly different between SLE group and control group.The relative risk suffered from SLE of T allele was 1.737 times higher than that of A allele (OR=1.737,95%CI:1.193～2.529),the serum level of IFN-γT allele carriers was significantly higher than that of no carriers (P<0.05).Conclusion IFN-γgene + 874 A/T polymorphism was associated with SLE,and T allele may be a risk factor for SLE.In which the IFN-γT allele carriers may be increase risk by enhancing the IFN-γexpression in the pathogenesis of SLE.