| T-2毒素、脱氧雪腐镰刀菌烯醇全抗原的合成及鉴定 |
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| 引用本文： | 张新,王丹,陆晶晶,周爽,赵云峰,吴永宁.T-2毒素、脱氧雪腐镰刀菌烯醇全抗原的合成及鉴定[J].卫生研究,2015(1):95-98,105. |
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| 作者姓名： | 张新 王丹 陆晶晶 周爽 赵云峰 吴永宁 |
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| 作者单位： | 国家食品安全风险评估中心卫生部食品安全风险评估重点实验室 |
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| 基金项目： | 国家自然科学基金(No.81102133) |
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| 摘 要： | 目的分别合成T-2毒素、脱氧雪腐镰刀菌烯醇(DON)的全抗原。方法采用琥珀酸酐法对分子结构进行修饰,产生易反应的官能团,并与载体蛋白偶联,从而具备免疫条件,用高性能基质辅助激光解吸电离-飞行时间质谱仪MALDI-TOF检测偶联物及偶联蛋白的分子量,用商品化的T-2和DON免疫试剂盒与偶联物进行棋盘滴定验证。结果T-2和DON的全抗原及BSA的分子量分别为67641.6、66680.4、66297.7,偶联物与免疫试剂盒中T-2和DON的抗体均呈阳性反应。结论合成的T-2、DON全抗原为相应抗体的制备和免疫学方法的建立奠定了基础。
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| 关 键 词： | T-2毒素 脱氧雪腐镰刀菌烯醇 全抗原 基质辅助激光解吸电离-飞行时间质谱仪 |
Preparation of characterization of complete antigen of T-2 toxin and deoxynivalenol |
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| ZHANG Xin;WANG Dan;LU Jingjing;ZHOU Shuang;ZHAO Yunfeng;WU Yongning.Preparation of characterization of complete antigen of T-2 toxin and deoxynivalenol[J].Journal of Hygiene Research,2015(1):95-98,105. |
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| Authors: | ZHANG Xin;WANG Dan;LU Jingjing;ZHOU Shuang;ZHAO Yunfeng;WU Yongning |
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| Affiliation: | ZHANG Xin;WANG Dan;LU Jingjing;ZHOU Shuang;ZHAO Yunfeng;WU Yongning;Key Laboratory of Food Safety Risk Assessment,National Center for Risk Assessment of Food Safety; |
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| Abstract: | Objective To synthesize the complete antigen of T-2 toxin and deoxynivalenol( DON),respectively. Methods T-2 toxin and DON were coupled with carrier protein after derivation with succinic anhydride. Molecular weights of the conjugates and carrier protein were detected by MALDI-TOF. And the performance of complete antigens were further validated by chequerboard titration using commercial immunoassay kits for T-2 toxin and DON. Results The molecular weights of T-2,DON conjugates and BSA were 67641. 6,66680. 4 and 66297. 7,respectively. Conclusion The preparation of complete antigen of T-2 and DON will greatly facilitated antibody production and immunoassay development. |
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| Keywords: | T-2 deoxynivalenol artificial antigen MALDI-TOF |
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