七氟醚对内毒素致急性肺损伤鼠肺泡毛细血管膜通透性的影响

目的　探讨七氟醚对内毒素致急性肺损伤鼠肺泡毛细血管膜通透性和肺泡灌洗液内炎性细胞的影响。方法　 4 8只Wistar大鼠 ,麻醉后静注伊万斯蓝 5 0mg/kg后 ,随机分为 4组 ,每组 12只。对照组 :股静脉注射生理盐水 1 2ml后机械通气 4h ;内毒素组 :股静脉注射内毒素 5mg/kg后机械通气 4h ;七氟醚 1L组和七氟醚 2L组 :股静脉注射内毒素 5mg/kg后机械通气 ,分别吸入肺泡气最低有效浓度 (MAC)为 1 0和 1 5的七氟醚 4h。 4h后取肺组织测定 :病理形态学积分 ,肺湿 /干重比 ,肺水含量 ,肺通透指数 ,伊万斯蓝含量和肺泡灌洗液内炎性细胞总数及百分比。结果　七氟醚1L组肺通透指数、伊万斯蓝含量、病理形态学积分分别由 4 6 8± 0 82 ,( 112 2 1± 11 4 4 )ng/mg ,9 17± 0 90下降到 3 98± 0 5 0 ,( 92 85± 11 80 )ng/mg ,7 5 0± 0 96 ;七氟醚 2L组下降到 3 91±0 34,( 96 33± 8 79)ng/mg ,7 6 7± 0 75。结论　 1 0和 1 5MAC七氟醚可降低内毒素所致急性肺损伤肺泡毛细血管膜通透性 ,使肺组织病理损伤减轻

Effects of sevoflurane on membrane permeability of alveolar capillaries in rats with acute lung injury caused by endotoxin

OBJECTIVE: To observe the effect of sevoflurane on membrane permeability of alveolar capillaries in rats with acute lung injury and the ratio of inflammatory cells in bronchoalveolar lavage fluid in rats with acute endotoxin lung injury. METHODS: 48 Wistar rats were selected and divided into group C, L, S1L and S2L after injection evans blue 50 mg/kg in random with 12 rats in each group. Group C was taken as control group, 1.2 ml normal saline was injected into the rats via femoral vein and then the rats were mechanically ventilated for 4 hours; The rats in group L were also mechanically ventilated for 4 hours after injection of endotoxin 5 mg/kg via the same vein. For the rats in group S1L and S2L, 1.0 or 1.5 minimal alveolar concentration (MAC) sevoflurane was inhaled with mechanical ventilation after injection of endotoxin 5 mg/kg. Evans blue was not injected into 6 rats of each group in order that the 6 rats could be used for pathological examination and alveoli lavage, lung pathomorphological score of the lung, lung wet/dry weight ratio, the content of lung water, lung permeability index, content of evans blue, total amount and ratio of inflammatory cells in bronchoalveolar lavage fluid (BALF) were all determined. RESULTS: Sevoflurane of 1.0 MAC and 1.5 MAC made lung wet/dry weight ratio and content of lung water change insignificantly; lung permeability index, content of evans blue and pathomorphological score in group S1L decreased from 4.86 +/- 0.82, 112.21 +/- 11.44 ng/mg, 9.17 +/- 0.90 to 3.98 +/- 0.50, 92.85 +/- 11.80 ng/mg, 7.50 +/- 0.96; group S2L decreased to 3.91 +/- 0.34, 96.33 +/- 8.79 ng/mg, 7.67 +/- 0.75. Sevoflurane of 1.0 MAC and 1.5 MAC did not have a significantly effect on total amount and ratio of inflammatory cells in BALF. CONCLUSION: Membrane permeability of alveolar capillaries after acute endotoxin lung injury decreased by inhalation of sevoflurane of 1.0 MAC and 1.5 MAC and pathological injury of lung tissue relieved.