| Development of simple sequence repeats (SSR) markers of ramie and comparison of SSR and inter-SSR marker systems |
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| 作者姓名： | ZHOU Jianlin JIE Yucheng JIANG Yanbo ZHONG Yingli LIU Yunhai ZHANG Jian |
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| 作者单位： | Department of Biochemistry and Molecular Biology,College of Life Science,Hunan Normal University,Changsha 410081,China,Institute of Bast Fiber Crops,Chinese Academy of Agricultural Science,Changsha 410006,China,Institute of Bast Fiber Crops,Chinese Academy of Agricultural Science,Changsha 410006,China,Department of Biochemistry and Molecular Biology,College of Life Science,Hunan Normal University,Changsha 410081,China,Department of Biochemistry and Molecular Biology,College of Life Science,Hunan Normal University,Changsha 410081,China,Department of Biochemistry and Molecular Biology,College of Life Science,Hunan Normal University,Changsha 410081,China |
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| 基金项目： | 国家自然科学基金，国家高技术研究发展计划(863计划)，湖南省自然科学基金 |
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| 摘 要： | Ramie (Boehmeria nivea L. ) is an important bast fiber crop. To study genetic background of this species, we isolated and characterized microsatellite markers of ramie. A genomic library containing inserts of rapid amplification of polymorphic DNA (RAPD)fragments was constructed, and screened by PCR amplification using anchored simple sequence repeats as primers. A total of 26 clones were identified as positives, and 13 microsatellite loci were found after sequencing. The polymorphism of these 13 microsatellite loci was examined and the utility of simple sequence repeats (SSR) and inter-SSR (ISSR) marker systems for genetic characterization compared using 19 selected ramie cultivars. Both approaches successfully discriminated the 19 cultivars which differed in the amount of polymorphism detected. The level of polymorphism detected by SSR was 95.0 %, higher than that by ISSR (72.3 % ), but the average polymorphism information content (PIC) of ISSR (0. 651) was higher than that of SSR (0. 441). The higher PIC value of ISSR suggests that ISSR is more efficient for fingerprinting ramie cultivars than SSR markers. However, because the SSR loci are codominant, they are more suitable for determining the homozygosity levels of ramie, constructing linkage map, quantitative trait loci study of complex traits and marker-as-sisted selection.
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| 关 键 词： | ramie (Boehmeria nivea L. Gaud) simple sequence repeats inter-simple sequence repeats |
Development of simple sequence repeats (SSR) markers of ramie and comparison of SSR and inter-SSR marker systems |
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| ZHOU Jianlin,JIE Yucheng,JIANG Yanbo,ZHONG Yingli,LIU Yunhai,ZHANG Jian.Development of simple sequence repeats (SSR) markers of ramie and comparison of SSR and inter-SSR marker systems[J].Progress in Natural Science,2005,15(2):137-142. |
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| Authors: | ZHOU Jianlin Jie Yucheng JIANG Yanbo ZHONG Yingli LIU Yunhai ZHANG Jian |
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| Affiliation: | 1. Department of Biochemistry and Molecular Biology,College of Life Science,Hunan Normal University,Changsha 410081,China
2. Institute of Bast Fiber Crops,Chinese Academy of Agricultural Science,Changsha 410006,China |
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| Abstract: | Ramie (Boehmeria nivea L. ) is an important bast fiber crop. To study genetic background of this species, we isolated and characterized microsatellite markers of ramie. A genomic library containing inserts of rapid amplification of polymorphic DNA (RAPD)fragments was constructed, and screened by PCR amplification using anchored simple sequence repeats as primers. A total of 26 clones were identified as positives, and 13 microsatellite loci were found after sequencing. The polymorphism of these 13 microsatellite loci was examined and the utility of simple sequence repeats (SSR) and inter-SSR (ISSR) marker systems for genetic characterization compared using 19 selected ramie cultivars. Both approaches successfully discriminated the 19 cultivars which differed in the amount of polymorphism detected. The level of polymorphism detected by SSR was 95.0 %, higher than that by ISSR (72.3 % ), but the average polymorphism information content (PIC) of ISSR (0. 651) was higher than that of SSR (0. 441). The higher PIC value of ISSR suggests that ISSR is more efficient for fingerprinting ramie cultivars than SSR markers. However, because the SSR loci are codominant, they are more suitable for determining the homozygosity levels of ramie, constructing linkage map, quantitative trait loci study of complex traits and marker-as-sisted selection. |
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| Keywords: | ramie (Boehmeria nivea L Gaud) simple sequence repeats inter-simple sequence repeats |
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