| 杜氏藻多糖的分离、纯化及结构分析 |
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| 引用本文： | 薛巧如,尹鸿萍,王旻,徐莹.杜氏藻多糖的分离、纯化及结构分析[J].药物生物技术,2003,10(2):96-99. |
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| 作者姓名： | 薛巧如 尹鸿萍 王旻 徐莹 |
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| 作者单位： | 中国药科大学生物制药学院,南京,210009 |
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| 摘 要： | 探讨杜氏藻中的糖复合物。采用热碱水提，乙醇酵沉，DEAE—32分离等方法分离纯化杜氏藻中的多糖复合物，对其进行理化测试并通过薄层层析，电泳，气相色谱对各组分进行定性定量分析。从杜氏藻中得到两种酸性多糖PDS1，PDS2，硫酸根含量分别为1．8％，6．0％；己糖醛酸含量为1．4％，19．2％。其中PDS1是带硫酸根的葡聚糖，PDS2是含葡萄糖，半乳糖，鼠李糖和甘露糖的酸性蛋白聚糖。
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| 关 键 词： | 杜氏藻多糖 分离 纯化 结构 分析 |
| 文章编号： | 1005-8915(2003)02-0096-04 |
Isolation, Purification and Chemical Properties of the Polysaccharides from D . Salina |
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| XUE Qiao-ru,YIN Hong-ping,WANG Min,XU Ying.Isolation, Purification and Chemical Properties of the Polysaccharides from D . Salina[J].Pharmaceutical Biotechnology,2003,10(2):96-99. |
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| Authors: | XUE Qiao-ru YIN Hong-ping WANG Min XU Ying |
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| Abstract: | The crude polysaccharide was isolated from D.Salin a after hot basic wa ter extraction. Two polysaccharides PDS 1, PDS 2 were purificated by DEAE-32 c hrom atography. Their components were identified by TLC, Agarose Electrophoresis, GC methods and so on. The result show that PDS 1 is a glucosan, and the PDS 2 is a s ulfated Proteoglycan which was composed of D-rhamnose,D-mannose, D-g alactose, D-rhamnose. |
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| Keywords: | D Salina Polysaccharides Structure Gasic chrom atography |
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