姜黄素对宫颈癌裸鼠移植瘤淋巴管生成及淋巴转移的影响

目的:探讨姜黄素对人宫颈癌裸鼠皮下移植瘤淋巴管生成及淋巴道转移的影响。方法:取对数生长期CaSki细胞接种于裸鼠右腋窝皮下，建立人宫颈癌裸鼠皮下移植瘤模型，免疫组化方法检测移植瘤组织中VEGF-C和VEGFR-3蛋白表达情况。结果:免疫组化显示姜黄素处理组的肿瘤组织VEGF-C、VEGFR-3蛋白表达水平较阴性对照组降低(P<0.05)。可见姜黄素能下调肿瘤细胞中VEGF-C和VEGFR-3蛋白的表达。结论:姜黄素具有抗宫颈癌淋巴管生成的作用,对宫颈癌淋巴道转移具有抑制作用,其主要机制与抑制VEGF-C和VEGFR-3蛋白的表达有关。

Experimental study on curcumin contributions to lymphangiogenesis and lymphatic me-tastasis in cervical carcinoma

Objective:To study the effects of curcumin on lymphangiogenesis and lymphatic metastasis of human cervical cancer subcutaneous xenograft in nude mice tube.Methods:To take the logarithmic growth phase of CaSki cells and inoculate them in nude mice right axillary subcutaneous，establish the model of human cervical carcinoma subcutaneous xenograft in nude mice and test VEGF-C and VEGFR-3 protein in transplanted tumor by immunohistochemical method.Results: Immunohistochemical method showed that compared with the control group，expressions of VEGF-C and VEGFR-3 in transplanted tumors were downregulated in curcumin groups．Curcumin can cut down the expression of VEGF-C and VEGFR-3 protein in tumor cells.Conclusion:Curcumin has a significant anti- lymphangiogenesis activity.Curcumin has an inhibitory effect on the cervical lymph node metastasis，which may be related with the inhibition expression of VEGF-C and VEGFR-3.