| 肾上腺髓质素缺失对单侧输尿管结扎小鼠MMP-1/TIMP-1表达的影响 |
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| 引用本文： | 王香婷,许庆友,丁英钧,王月华,王筝,谷艳丽,张磊磊.肾上腺髓质素缺失对单侧输尿管结扎小鼠MMP-1/TIMP-1表达的影响[J].中国老年学杂志,2010,30(6). |
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| 作者姓名： | 王香婷 许庆友 丁英钧 王月华 王筝 谷艳丽 张磊磊 |
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| 作者单位： | 1. 河北医科大学中医学院中西诊断学教研室,河北,石家庄,050091
2. 河北医科大学中西医结合学院内科学教研室 |
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| 基金项目： | 河北省科技厅资助项目 |
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| 摘 要： | 目的 探讨肾上腺髓质素在肾间质纤维化实验动物肾脏基质金属蛋白酶-1/基质金属蛋白酶抑制剂-1(MMP-1/TIMP-1)表达中的作用.方法 结扎肾上腺髓质素基因敲除小鼠及野生小鼠单侧输尿管,制备肾间质纤维化实验动物模型并给予缬沙坦灌胃治疗,采用免疫组化、原位杂交、RT-PCR方法检测MMP-1及TIMP-1的表达.结果 单侧输尿管结扎 (UUO) 小鼠肾脏MMP-1的表达减弱,而TIMP-1的表达显著上调,基因敲除小鼠较野生小鼠更为明显,采用缬沙坦治疗可部分抑制TIMP-1的表达.结论 肾上腺髓质素可以通过纠正MMP-1/TIMP-1的失衡,对UUO介导的肾间质纤维化起到保护作用.
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| 关 键 词： | 肾上腺髓质素 单侧输尿管结扎 基质金属蛋白酶-1 基质金属蛋白酶抑制剂-1 |
Expression of MMP-1/TIMP-1 in adrenomedullin knockout mice with unilateral ureteral obstruction |
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| WANG Xiang-Ting,XU Qing-You,DING Ying-Jun,et al..Expression of MMP-1/TIMP-1 in adrenomedullin knockout mice with unilateral ureteral obstruction[J].Chinese Journal of Gerontology,2010,30(6). |
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| Authors: | WANG Xiang-Ting XU Qing-You DING Ying-Jun |
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| Affiliation: | WANG Xiang-Ting,XU Qing-You,DING Ying-Jun,et al.Teaching , Research Room of Traditional Chinese Medical , Western Medical Diagnosis,College of Traditional Chinese Medicine,Hebei Medical University,Shijiazhuang 050091,Hebei,China |
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| Abstract: | Objective To investigate the effect of adrenomedullin on expression of MMP-1/TIMP-1 in adrenomedullin knockout (AMKO) and wild type (WT) mice with unilateral ureteral obstruction (UUO).Methods Renal interstitial fibrosis model was made with UUO in AMKO and WT mice and treated with valsartan by oral.The expressions of matrix metalloproteinases-1 (MMP-1) and tissue inhibitor of metalloproteinases-1 (TIMP-1) were examined with immunohistochemistry,in site hybridization and RT-PCR.Results There was the unbalanc... |
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| Keywords: | Adrenomedullin Unilateral ureteral obstruction Matrix metalloproteinases-1 Tissue inhibitor of metalloproteinases-1 |
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