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| 双水相萃取直接从枯草芽孢杆菌发酵液中提取β-甘露聚糖酶 | |
| 引用本文： | 余红英,孙远明,王炜军,杨幼慧,杨跃生.双水相萃取直接从枯草芽孢杆菌发酵液中提取β-甘露聚糖酶[J].化学世界,2003,44(11):569-571,574. |
| 作者姓名： | 余红英 孙远明 王炜军 杨幼慧 杨跃生 |
| 作者单位： | 1. 华南农业大学食品学院,广东,广州,510642;广东工业大学轻化工学院,广东,广州,510090 2. 华南农业大学食品学院,广东,广州,510642 3. 华南农大生命科学学院 |
| 摘 要： | 研究了聚乙二醇(PEG)平均相对分子质量、PEG浓度、(NH4)2SO4浓度、NaCl浓度对β-甘露聚糖酶和总蛋白分配系数、相体积比和萃取率的影响。实验表明PEG100020%(m/m)和(NH4)2SO415%(m/m),NaCl为2%(m/m)组成的双水相体系,室温下直接对含菌体的枯草芽孢杆菌发酵液抽提β-甘露聚糖酶,可纯化2.76倍,萃取率达98.79%。 |
| 关 键 词： | 双水相体系 β-甘露聚糖酶 提取 |
| 文章编号： | 0367-6358(2003)11-0569-03 |
Studies on the Extraction of β-Mannanase by PEG/(NH4)2SO4 Aqueous Two-phase System |
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| Abstract: | The effects of the relative molecular mass and concentration of PEG and the concentration of (NH4)2SO4 and NaCl on the partition coefficient of β mannanase and the total protein and the volume ratio of the top phases were studied. β mannanase was recovered with high yield in the top phase when 20%(m/m) PEG, 15%(m/m) and 2%(m/m) NaCl were applied. The purity of β mannanase increased by 2.76 times with a yield of 98.79%. |
| Keywords: | aqueous two-phase system β-mannanase extraction |
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