cDNA芯片检测棉花纤维发育相关基因的表达

利用cDNA芯片技术分析E6、Lipid transfer protein(LTP)、Proline-rich protein(PRP）、Expansin、Tubulin、Annexin等家族的63个棉花纤维发育相关基因，在陆地棉徐州-142开花后10d纤维组织及其无长绒无短绒突变体开花后10d胚珠中的表达差异，发现属于E6、LTP、PRP、Expansin家族的基因在两种组织中存在显著的表达差异，符合前人的报道。其中E6、LTP家族的基因在两种组织中的表达差异最大，个别基因表达差异甚至达到15倍之多。而Tubulin家族的基因在两种组织中的表达差异不大，检测的24个Tubulin家族基因中，仅2个基因在纤维组织和胚珠中存在显著表达差异。cDNA芯片技术可以高通量鉴定棉花纤维相关基因以及研究棉花发育相关基因的表达谱。

Analysis of Genes Expression during Cotton Fiber Development by cDNA Microarray

To study cotton fiber development-associated gene expression, we prepared a cDNA microarray containing 63cDNAs, including E6, LTP, PRP, Expansin, Tubulin and Annexin, and used total RNAs extracted from 10DPA (days post anthesis) cotton fiber as well as from 10DPA ovule of fuzz-less and lint-less mutant. Differential expression of E6, LTP, PRP and Expansin gene families, especially that of ? and LTP gene families, were observed from 10DPA cotton fiber and 10DPA mutant. Our analyses are in accordance with previous results. The current work confirms the reliability of using cDNA microarray to detect cotton fiber development-assotiated genes.