Accelerator Analysis of Tributyltin Adsorbed onto the Surface of a Tributyltin Resistant Marine Pseudoalteromonas sp. Cell |
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| Authors: | Haruo Mimura Ryusei Sato Yu Sasaki Yuichi Furuyama Akira Taniike Kazutoshi Yoshida and Akira Kitamura |
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| Affiliation: | 1. Graduate School of Maritime Sciences, Kobe University, 5-1-1, Fukae, Kobe 658-0022, Japan. E-Mails: (R. S.); (Y. S.); (Y. F.); (A. T.); (A. K.);2. Hyogo Prefectural Institute of Technology, 3-1-12, Yukihira, Kobe 654-0037, Japan. E-Mail: (K. Y.) |
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| Abstract: | Tributyltin (TBT) released into seawater from ship hulls is a stable marine pollutant and obviously remains in marine environments. We isolated a TBT resistant marine Pseudoalteromonas sp. TBT1 from sediment of a ship’s ballast water. The isolate (109.3 ± 0.2 colony-forming units mL?1) adsorbed TBT in proportion to the concentrations of TBTCl externally added up to 3 mM, where the number of TBT adsorbed by a single cell was estimated to be 108.2. The value was reduced to about one-fifth when the lysozyme-treated cells were used. The surface of ethanol treated cells became rough, but the capacity of TBT adsorption was the same as that for native cells. These results indicate that the function of the cell surface, rather than that structure, plays an important role to the adsorption of TBT. The adsorption state of TBT seems to be multi-layer when the number of more than 106.8 TBT molecules is adsorbed by a single cell. |
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| Keywords: | Accelerator analysis tributyltin anti-fouling agent adsorption cell surface Pseudoalteromonas sp TBT1 scanning electron microscope |
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