主动免疫治疗原因不明习惯性流产后细胞免疫功能的变化

目的:探讨原因不明习惯性流产(URSA)患者外周血T淋巴细胞亚群在主动免疫治疗前后的变化。`方法:用流式细胞仪测定并比较经主动免疫治疗前后的32例URSA患者外周血CD3+、CD4+、CD8+细胞亚群百分率及CD4+/CD8+比值;同时选择30例正常早孕妇女作为对照。结果:与对照组比较,URSA患者外周血中CD4+细胞百分率及CD4+/CD8+比值升高,CD8+细胞百分率降低(均P<0.01),而两组CD3+细胞百分率无差异。主动免疫治疗后CD3+细胞无变化,CD4+细胞、CD4+/CD8+比值较治疗前明显下降;CD8+细胞则明显升高(均P<0.01)。结论:CD4+细胞百分率升高、CD8+细胞百分率降低与UR-SA发生有关;主动免疫治疗可降低CD4+细胞百分率,提高CD8+细胞百分率,有利于妊娠发展。

The Changes of T Lymphocytes Sub-types in Unexplained Recurrent Spontaneous Abortion Women after Active Immunotherapy.

Objective:To investigate the changes of T lymphocytes sub-types in unexplained recurrent spontaneous abortion(URSA) women before and after active immunotherapy.Methods:Flow cytometry was used to measure T lymphocytes sub-types of 32 URSA women before and after active immunotherapy and 30 normal early pregnant women were taken as control.Results:Compared with control group,CD4 cells and CD4 /CD8 ratio increased significantly in URSA women,but CD8 cells decreased significantly,there were no significant changes in CD3 cells between two groups.After active immunotherapy,CD4 cells and CD4 /CD8 ratio dropped,but CD8 cells increased greatly in URSA women,there were still no significant changes in CD3 cells.Conclusion:The occurrence of URSA might be associated with proliferation of the CD4 cells and decrease of the CD8 cells.Markedly dropped CD4 cells and increased CD8 cells in URSA women after active immunotherapy is in favor of conception.