急性ITP患儿外周血中CD4^＋CD25^＋Foxp3^＋Tr细胞、IL-7水平变化及意义

目的探讨急性特发性血小板减少性紫癜（AITP）的发病机制。方法选择35例AITP患儿（AITP组）和30例健康查体者（对照组），用流式细胞仪检测外周血CD4^＋CD25^＋Foxp3^＋调节性T（Tr）细胞数量及占CD4^＋细胞比例，ELISA法检测血浆中IL-7水平。结果AITP组和对照组外周血CD4^＋CD25^＋Foxp3^＋Tr细胞／CD；细胞分别为0．11±0．04、0．15±0．02，IL-7分别为（2．32±0．53）、（0．44±0．80）pg／ml，P均〈0．05；AITP组外周血IL-7水平与CD4^＋CD25^＋Foxp3^＋Tr细胞／CD；细胞呈负相关（r=0．71，P〈0．05）。结论CD4^＋CD25^＋Foxp3^＋Tr细胞数量减少、IL-7水平升高可能在AITP发病中具有重要作用，机制为降低有效免疫抑制作用，导致自身反应性T细胞激活增多、凋亡减少，促进血小板破坏。

Changes and significance of CD+4CD+25Foxp3+ Tr cells and IL-7 level in peripheral blood of children with acute idiopathic thrombocytopenic purpura

Objective To investigate the pathogenesis of acute idiopathic thrombocytopenic purpura（AITP）. Methods 35 cases with AITP （ AITP group） and 30 healthy cases （ control group） were selected, the quantity of the CD4^＋CD25^＋Foxp3^＋ Tr cells and proportion of CD4^＋CD25^＋Foxp3^＋Tr cells/CD4^＋ cells were detected by flow cytometry, and the level of IL-7 were detected by ELISA. Results The proportion of CD4^＋CD25^＋Foxp3^＋ Tr cells/CD4^＋ cells in AITP group and control groupwere 2.96% ± 1.05% and 5.37% ± 0.69% （ P 〈 0.05 ） , while the level of IL-7 were （ 2.32 ±0. 53） and （0.44 ± 0.80）pg/ml（P 〈0.05 ）. The level of IL-7 in AITP group was negatively correlated with the proportion of the CD4^＋CD25^＋Foxp3^＋ Tr cells / CD4^＋ cells （ r = 0.71,P 〈 0.05）. Conclusion The quantity reduction of CD4^＋CD25^＋Foxp3^＋Tr ceils and up-regulation of IL-7 may play important role in the pathogenesis of AITP ,the mechanism maybe the the function weakness of immunosuppression.