血管内皮细胞生长因子121(VEGF121)在胃癌中的表达及其临床意义

目的　探讨VEGF12 1血管生成因子在胃癌中的表达与胃癌微血管密度及胃癌临床病理特征的关系。方法　采用免疫组织化学方法对 5 9例胃癌、2 5例胃慢性良性病变、15例癌旁正常胃黏膜组织检测VEGF12 1阳性表达及微血管密度 (CD34标记 )。结果　 (1)胃癌中VEGF12 1阳性表达率为 79 6 6 % (4 7/5 9) ,高于胃慢性良性病变和癌旁正常组织 (P <0 0 5 ) ;胃癌微血管密度 (MVD)高于胃慢性良性病变和癌旁正常组织 (P <0 0 5 )。 (2 )胃癌中VEGF12 1阳性表达率在不同浸润深度组和有无远处转移组有显著性差异 (P <0 0 5 )。胃癌中微血管数量增加 ,形态结构异常 (P <0 0 5 )。结论　VEGF12 1在胃癌中高表达 ,并与胃癌的浸润转移一致。检测VEGF12 1在胃癌中的表达对判断胃癌的恶性程度有价值。

The expression and clinical significance of vascular endothelial growth factor 121 (VEGF121) in gastric carcinoma

Objective To investigate the expression of angiogenic factors VEGF 121 in gastric carcinoma ,tumour microvessel density (MVD) immunoreactivity of gastric carcinoma and whether clinicopatholgical features of gastric carcinoma are related to the expression of VEGF 121 .Methods Immunohistochemistry technique (SP or SABC) was used to test the expression of the angiogenic factor VEGF 121 and CD34 in 59 gastric carcinoma specimens,15 normal gastric membrane beside carcinoma specimens and 25 gastric benign lesions specimens embedded in paraffin wax.Results (1) Immunoreactivity of VEGF 121 was found in cytoplastic and cell surface of most gastric carcinoma cell (positive staining 47 in 59), the rate of positive staining in gastric carcinoma was the highest (P<0.05); the MVD of gastric carcinoma was higher than that of normal gastric tissue beside carcinoma and that of gastric benign lesions (P<0.05), Moreover, the shape and structure of microvessel was abnormal. (2)The expression of VEGF 121 vavies with different infiltration depth and with or without distant metastasis(P<0.05).Conclusion Infiltrating depth and distantant metastasis are positively related to the expression of VEGF 121 . VEGF 121 expression may be useful to evaluate clinical progression of gastric carcinoma.