SM22α对结直肠癌细胞侵袭和迁移能力的影响

目的:探究平滑肌22α蛋白(SM22α)对结直肠癌细胞侵袭和迁移能力的影响,并探讨其分子机制。方法:通过慢病毒转染人结直肠癌细胞HCTL16构建SM22α过表达细胞,应用细胞划痕实验检测细胞迁移能力的变化;Transwell小室检测细胞侵袭和迁移能力的变化;使用RT-qPCR检测细胞SM22αmRNA表达的改变;通过Western blot法检测细胞外信号调节激酶(ERK)、p-ERK、基质金属蛋白酶9(MMP-9)和SM22α蛋白水平。结果:成功构建HCT116过表达SM22α细胞;SM22α过表达细胞的侵袭和迁移能力减弱(P0.05);SM22α过表达抑制p-ERK和MMP-9的蛋白水平(P0.05)。结论:SM22α通过抑制ERK/MMP-9信号通路调节结直肠癌细胞侵袭和迁移能力。

Effects of SM22α on migration and invasion abilities of colorectal cancer cells

AIM:To explore the effects of smooth muscle 22α protein (SM22α) on the migration and invasion abilities of colorectal cancer cells, and to investigate its molecular mechanism. METHODS:The SM22α-over-expressing cells were constructed by lentivirus transfection. The cell migration ability was detected by wound healing assay. The changes of cell migration and invasion abilities were measured by Transwell assay. RT-qPCR was used to detect the changes of SM22α mRNA level. The protein levels of extracellular signal-regulated kinase (ERK), p-ERK, matrix metalloproteinase-9 (MMP-9) and SM22α were determined by Western blot. RESULTS:HCT116 cells with SM22α over-expression were constructed successfully. SM22α inhibited the migration and invasion abilities of colorectal cancer cells. SM22α over-expression decreased the protein levels of p-ERK and MMP-9 (P<0.05).CONCLUSION:SM22α inhibits the migration and invasion abilities of colorectal cancer cells by inhibiting ERK/MMP-9 signaling pathways.