槐榆煎剂对肛肠手术后大鼠血清中TNF-α、IL-6和IL-10含量的影响

目的:观察槐榆煎剂对肛肠手术后大鼠血清中肿瘤坏死因子α(TNF-α)、白细胞介素-6(IL-6)和白细胞介素-10(IL-10)含量的影响,初步研究其作用机制。方法:40只Sprague-Dawley大鼠随机分为正常对照组、模型组以及槐榆煎剂低、高剂量组,每组10只,酶联免疫吸附法(ELISA)检测各组大鼠血清中TNF-α、IL-6和IL-10的含量;HE染色法检测肛周组织的病理学变化;Western blotting法检测肛周组织中细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)、血管细胞黏附分子-1(vascular cell adhesion molecule-1,VCAM-1)和NF-κB的蛋白表达。结果:给予槐榆煎剂干预后,可抑制肛肠手术后大鼠血清中TNF-α、IL-6含量的升高与IL-10含量的降低,减轻大鼠肛周组织的水肿及充血程度,抑制肛肠手术后ICAM-1、VCAM-1和NF-κB蛋白表达的上调。结论:槐榆煎剂可调控血清中TNF-α、IL-6与IL-10的含量,并影响肛周组织中ICAM-1、VCAM-1和NF-κB的表达。

Effects of Huaiyu decoctum on serum concentrations of TNF-α, IL-6 and IL-10 in rats after anorectal operation

ATM: To investigate the effects of Huaiyu decoctum on the serum concentrations of TNF-α, IL-6 and IL-10 in rats after anorectal operation. METHODS: Sprague-Dawley rats (n=40) were randomly divided into 4 groups:normal group, model group, low-dose Huaiyu decoctum group and high-dose Huaiyu decoctum group. The concentrations of TNF-α, IL-6 and IL-10 in the rat serum were measured by ELISA. The pathologic changes of the anorectal tissues were observed under microscope with HE staining. The protein expression of ICAM-1, VCAM-1 and NF-κB was determined by Western blotting. RESULTS: After Huaiyu decoctum administration, TNF-α and IL-6 concentrations in the serum were significantly decreased, and IL-10 concentration was increased as compared with model group. Moreover, Huaiyu decoctum markedly attenuated edema and hyperemia in the rats after anorectal operation. The protein expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and NF-κB in the anorectal tissues was obviously inhibited by Huaiyu decoctum treatment. CONCLUSION: Huaiyu decoctum improves the recovery of anorectal tissues after operation by decreasing the serum concentrations of TNF-α, IL-6 and IL-10, and reducing the protein expression of ICAM-1, VCAM-1 and NF-κB in the anorectal tissues.