不同萃取纤维在半枝莲挥发性成分分析中的应用

目的: 分析比较3种不同固相萃取纤维头(PA,PDMS,PDMS-DVB)对半枝莲挥发性成分的吸附性。方法: 采用PA,PDMS,PDMS-DVB SPME萃取头,与气相色谱-质谱联用,分析半枝莲挥发性成分,并用归一化法计算各成分相对含量。结果: 共鉴别出90个化合物。PA纤维头共鉴定出41个化合物,占总峰面积的83.61%。PDMS纤维头共鉴定出40个化合物,占总峰面积的94.63%。PDMS-DVB纤维头共鉴定出53个化合物,占总峰面积的89.10%。3种纤维头共同检出化合物13个。结论: 不同纤维头吸附的挥发性成分有较大差异。PDMS对烷烃及酮类化合物有较高选择性;纤维头PA对酸类和酮类化合物有较高选择性;纤维头PDMS-DVB对萘类、烷烃、烯烃和酮类化合物有较高选择性。

Analysis of Volatile Compounds in Scutellaria barbata Using Different Types of Fibers

Objective: To analysis of the volatile compounds in Scutellaria barbata using three types of fibers including PA, PDMS and PDMS-DVB. Method: A headspace solid-phase microextraction (HS-SPME) method followed by gas chromatography-mass spectrometry (GC-MS) is described for the analysis of volatile compounds in S. barbata using three types of fibers. The volatile compounds of S. barbata were extracted by PA, PDMS and PDMS-DVB fiber individually. The relative content of each component was calculated by the normalization method. Result: 90 volatile compounds were found in S. barbata., using three types of fibers. There were 41 volatile compounds (83.61%) identified in PA extract;40 volatile compounds (94.63%) identified in PDMS extract;53 volatile compounds (89.10%) identified in PDMS-DVB extract. Among them, 13 compounds were common peaksin three types of fibers. Conclusion: The results made by the three types of fibers are quite different. more alkanes and ketones can be identified for PA, more acids and ketones can be identified for PDMS, more naphthalenes, alkanes, olefinsand ketones can be identified for PDMS-DVB.