| 重组RTB蛋白对巨噬细胞活化及对NF—κB信号通路的影响 |
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| 引用本文： | 李松岩,许娜,刘洋,肖世峰,刘林娜,万家余,张建营,孙成彪,周铁忠,刘文森.重组RTB蛋白对巨噬细胞活化及对NF—κB信号通路的影响[J].兽医大学学报,2014(1):83-86. |
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| 作者姓名： | 李松岩 许娜 刘洋 肖世峰 刘林娜 万家余 张建营 孙成彪 周铁忠 刘文森 |
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| 作者单位： | [1]吉林农业大学动物科学技术学院,吉林长春130118 [2]吉林医药学院,吉林吉林132000 [3]军事医学科学院军事兽医研究所吉林省人兽共患病防控重点实验室,吉林长春130122 [4]辽宁医学院动物医学院,辽宁锦州121001 |
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| 基金项目： | 国家自然科学基金资助项目（31372478） |
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| 摘 要： | 采用重组RTB蛋白刺激RAW264．7细胞，对iNOS、IL-6和TNFJamRNA表达及IκB-α和NF-κBp65磷酸化蛋白表达进行分析，探讨重组RTB蛋白对巨噬细胞活化及对NF-xB信号通路的影响。结果显示，重组RTB蛋白组RAW264．7细胞iNOS、IL-6和TNF—αmRNA的表达显著高于对照组（P〈0．01），并呈现时间和计量依赖性；加入NF—κB抑制剂BAY后，iNOS、IL-6和TNF0amRNA表达降低（P〈0．05或P〈0．01）。随RTB刺激RAW264．7时间的延长，IκB-α蛋白的磷酸化程度增强，NF-κBp65磷酸化蛋白表达逐渐增高，表明重组RTB蛋白具有促进巨噬细胞活化及活化NF-κB信号通路的功能。
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| 关 键 词： | 重组RTB 巨噬细胞 信号通路 |
Effect of the recombinant RTB on the activation of macrophages and the NF-κB signaling pathway |
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| Authors: | LI Song-yan XU Na LIU Yang XIAO Shi-feng LIU Lin-na WAN Jia-yu ZHANG Jian-ying SUN Cheng-biao ZHOU Tie-zhong LIU Wen-sen |
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| Affiliation: | 1. College of Animal Science and Technology , Jilin Agricuhural University, Changchun 130118, China; 2. Jilin Medical College, Jilin, Jilin 132000, China ; 3. Key Laboratory of Jilin Province for Zoonosis Prevention and Control, The Military Veterinary Institute ,Academy of Military Medical Science, Changchun 130122, China ; 4. College of Animal Medicine ,Liaoning Medical College ,J inzhou,Liaoning 121001 ,China) |
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| Abstract: | To explore the role of RTB recombinant protein on macrophage activation and the influ- ence of NF-κB signaling pathway, the expression of iNOS,IL-6 and TNF-α mRNA and IκB-α and expression of NF-κBp65 phosphorylated protein were analyzed by the recombinant RTB stimula- ting RAW264.7 cells. The results showed that the expression of recombinant protein group RTB RAW264.7 cells iNOS.IL-6 and TNF-α mRNA was significantly higher than the contril （P〈 0.01）, and showed time and measurement dependence ; after adding NF-κB inhibitor BAY, expression of iNOS.IL-6 and TNF-α mRNA decreased（P〈0.05 or P;0.01）. As the time extended that RTB stimulated RAW264.7 ,IκB-a protein phosphorylation level enhanced, and the expression of NF-κBp65 phosphorylated protein increased gradually,indicating that the recombinant protein can promote macrophage activation and activate NF-κB signaling pathway. |
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| Keywords: | recombinant RTB macrophages signal transduction pathway |
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