| 孕妇外周血PCR技术用于胎儿产前性别诊断的可靠性分析 |
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| 引用本文： | 邱曙东,蒋三亮,常姣娥.孕妇外周血PCR技术用于胎儿产前性别诊断的可靠性分析[J].西安交通大学学报(医学版),1995(1). |
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| 作者姓名： | 邱曙东 蒋三亮 常姣娥 |
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| 作者单位： | 西安组织胚胎学教研室 |
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| 摘 要： | 采用多聚酶链反应（ＰＣＲ）技术，以Ｙ染色体特异重复序列Ｙ１．１、Ｙ１．２和ＳＲＹ基因编码区Ｐ１、Ｐ２为引物，分析了用孕妇外周血进行胎儿性别诊断的可靠性，并与绒毛样本进行了对比。结果表明：用孕妇外周血时，Ｙ１．１，Ｙ１．２引物的诊断符合率为７５％；Ｐ１、Ｐ２引物不能扩增出Ｙ特异ＤＮＡ序列。而用绒毛样本时，两种引物的诊断符合率均为１００％。结果提示：孕妇外周血ＰＣＲ技术用于胎儿性别诊断尚有待改进，而采用绒毛样本进行胎儿性别诊断更为可靠。
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| 关 键 词： | 孕妇外周血 胎儿性别诊断 聚合酶链反应 |
A RBLIABILITY ANALYSIS OF FETAL SEX DETERMINATION WITH MATERNAL BLOOD BY POLYMORASE CHAIN REAACTION |
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| Qiu Shudong, Jiang Sanliang, Chang Jiaoe.A RBLIABILITY ANALYSIS OF FETAL SEX DETERMINATION WITH MATERNAL BLOOD BY POLYMORASE CHAIN REAACTION[J].Journal of Xi‘an Jiaotong University:Medical Sciences,1995(1). |
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| Authors: | Qiu Shudong Jiang Sanliang Chang Jiaoe |
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| Affiliation: | Department of Histology and Embryolcgy |
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| Abstract: | Using Y-specific repeat primer Y1.1,Y1.2 and encoded region primer P1, P2 of SRY gene, the reliability of fetal sex determination was analyzed with meternal blood by polymorase chain reaction, and compared with chrionic villi. The results showed tbat with the use of maternal blood samples, diagnostic accordance rate of Y1.1, Y1. 2 primer was 75%, P1, P2 primer failed to amplify Y-DNA.But with the use of chrionic villi samples, the diagnostic accordonce rates of both primers were 100%.The results suggest that fetal sex determination with maternal blood by polymorase chain reaction needs to be further improved, but that fetal sex determination with chrionic villi samples is much more reliable. |
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| Keywords: | maternal blood fetal sex determination polymorase chain reaction |
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