香港远志提取物对局灶性脑缺血大鼠脑保护作用与作用机制

目的探讨香港远志提取物对局灶性脑缺血大鼠脑保护作用与机制。方法线栓法制备SD大鼠大脑中动脉闭塞(MCAO)模型,随机分4组:假手术组、模型组及治疗A、B组。治疗组按设定方式给药,假手术组、模型组给1%吐温溶液。观察术后24h神经功能缺损评分(NBDS)、脑梗死体积(IV)、血清神经元烯醇化酶(NSE)及神经元凋亡、Bcl-2、Bax表达。结果与假手术组比较,模型组和治疗组NBDS、IV、NSE、神经元凋亡及Bcl-2、Bax显著增高、Bcl-2/Bax显著降低(P<0.05)。与模型组比较,治疗组NBDS、IV、NSE及神经元凋亡、Bax显著降低、Bcl-2、Bcl-2/Bax显著增高(P<0.05),治疗组间数据亦有显著差异(P<0.05)。结论香港远志提取物预处理,对局灶性脑缺血大鼠脑神经有保护作用,机制可能是促进Bcl-2、抑制Bax表达,上调Bcl-2/Bax比值,阻止神经元凋亡。

Neuroprotective effect and mechanism of polygala hongkongensis hemsl extracts on the focal cerebral ischemia in rats

Objective To investigate the neuroprotective effect and mechanism of polygala hongkongensis hemsl extracts(PHHE)on the focal cerebral ischemia in rats.Methods SD rats were randomly divided into 4 groups,sham operated group,model group and treatment group A and B.Treatment groups received drug respectively according to grouping protocol,the sham operated group and model group received 1% tween-80 solution.The middle cerebral artery occlusion(MCAO)was made by thread approach,at 24h after MCAO,the neurological behavior deficits scores(NBDS),infarction volume(IV),NSE and neuronal apoptosis and expressions of Bcl-2 and Bax were observed.Results Compared with sham operated group,the NBDS,IV,NSE,neuronal apoptosis and expressions of Bcl-2 and Bax were significantly increased and the Bcl-2/Bax was significantly decreased in both the model and treatment groups(P<0.05).Compared with model group,the NBDS,IV,NSE,neuronal apoptosis and Bax were significantly decreased and Bcl-2 and Bcl-2/Bax were significantly increased in treatment groups(P<0.05).The data between treatment groups showed significantly difference(P<0.05).Conclusions PHHE given prior to the focal cerebral ischemia plays a neuroprotective role which may be achieved by increasing the expression of Bcl-2,decreasing that of Bax,increasing Bcl-2/Bax and inhibiting neuronal apoptosis.