慢性酒精摄入对大鼠脂肪组织胰岛素敏感性影响

目的观察不同剂量酒精对雄性大鼠脂肪组织胰岛素敏感性的影响,并研究其可能的分子机制。方法用不同浓度(v/v)的酒精灌胃19 w,检测大鼠血清中胰岛素、葡萄糖含量,计算胰岛素抵抗程度;采用RT-PCR检测脂肪组织中磷脂酰肌醇-3-磷酸激酶(PI3K)mRNA、葡萄糖转运体4(GLUT4)mRNA表达水平。结果与对照组相比,中、高剂量组空腹葡萄糖浓度升高有统计学意义(P<0.05),低、中剂量组胰岛素浓度升高有统计学意义(P<0.05);各剂量组胰岛素抵抗指数(HOMA-IR)的升高均有统计学意义(P<0.05)。各剂量组的PI3K mRNA和GLUT4 mRNA表达均升高。结论长期过量酒精摄入引起胰岛素抵抗,引起脂肪组织胰岛素信号转导途径中磷PI3K mRNA和GLUT4 mRNA的表达升高。

Study on the effect of ethanol on the insulin sensitivity of adipose tissue in rats

Objective To investigate the effect of ethanol on the insulin sensitivity of adipose tissue in rats and explore its possible molecular mechanisms.Methods The rats were administered different concentrations of alcohol,after 19 weeks the concentrations of serum insulin and glucose were determined.Furthermore,levels of PI3K mRNA and GLUT4 mRNA expression in the adipose tissue were detected and the insulin resistance index(HOMA-IR) were calculated.Results Compared with control group,the glucose concentration significantly increased in the middle and high alcohol groups,respectively((P<0.05));the insulin concentration significantly increased in the low and middle alcohol groups((P<0.05));the HOMA-IR significantly increased in all ethanol groups((P<0.05)).Moreover,the levels of PI3K mRNA and GLUT4 mRNA expression were remarkably enhanced in all groups.Conclusions Chronic excessive ethanol intake could induce insulin resistance,enhance the expression of PI3K mRNA and GLUT4 mRNA in the insulin signaling transduction pass in the adipose tissue.