AdCMVCD／5FC自镣基因系统治疗CABG术后再狭窄

目的 研究胞嘧啶脱氨酶基因/5—氟胞嘧啶自杀基因系统对冠状动脉旁路移植术后再狭窄的治疗作用。 方法将含AdCMVCD的病毒上清加压注入兔颈外静脉管腔,半小时后剪下该静脉并用Cuff技术移植于兔颈动脉上,以单纯移植组和AdCMVLacz为对照,通过光镜和电镜观察平滑肌细胞的形态变化,并对血管桥外径、管腔、内膜、中膜面积和外弹力膜周长及管腔相对丧失率进行定量分析,以观察抑制内膜和中膜增生、防止血管再狭窄的效果。 结果 治疗组的内膜和中膜面积明显小于两个对照组;管腔丧失率亦明显减少,AdCMVCD组为3.68±0.42％,单纯移植组为9.68±0.41％.P<0.01。结论 AdCMVCD自杀基因系统对冠状动脉旁路移植术后再狭窄有明显的治疗作用。

Treatment for restenosis of vein grafts using the system including a replication-defective recombi- nant adenovirus vector containing CMV promoter and Escherichia coll cytosine deaminase gene

Objective To evaluate the therapy effect of CD/5 -FC suicide gene system on the restenosis after the operation of coronary artery vascular by-pass graft. Methods The supernatant containing AdCMVCD is injected to the rabbit external jugular vein after upstream and occlusion downstream.After 30 min,this vein segment was implanted to the carotid of common side eusing the cuff technique.The untreated vein graft and Ad-CMVlacz transfected vein graft were used as control.Four-weeks later the vein grafts were isolated and the transfection efficiency were evaluated by RT-PCR and the quantification of the activity of (3-Lac.Morphological changes of the SMC were observed under light microscope and transmission electron! microscope.The diameter,area of vessel cavities,intima and media,were assayed quantitatively and the relative cavity lose rates were calculat-ed.The inhibition effects on the intima and media were accessed using statistics methosds. Results Gene transfection with adenoviral vector is very effective and reliable.Comparing with the control group,the area of intima and media of the AdCMVCD group is significant small and the relative cavity lose rates decreased. (AdCMVCD group3.68+ 0.42%);control group,9.68+0.4%,P<0.01).The relative cavities lose rate,area of intima and media of the AdCMVLacz group is higher than the control group. Conclusions Our experiment shows that Adc MVCD suicide gene therapy system is so effective that is can be used to treat the restenosis of the coronary artery bypass graft.