| 枯否细胞条件培养基及混合血清对肝星状细胞HSC-T6的刺激作用 |
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| 引用本文： | 吴强,杨雁,张宵翔,薛绍礼,邹宇宏,陈敏珠.枯否细胞条件培养基及混合血清对肝星状细胞HSC-T6的刺激作用[J].安徽医科大学学报,2004,39(1):9-12. |
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| 作者姓名： | 吴强 杨雁 张宵翔 薛绍礼 邹宇宏 陈敏珠 |
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| 作者单位： | 安徽医科大学病理学教研室,合肥,230032;安徽医科大学临床药理研究所,合肥,230032;安徽医科大学细胞工程学教研室,合肥,230032 |
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| 摘 要： | 目的探讨枯否细胞条件培养基(KCCM)、混合的新生牛血清(NBS)和大鼠血清(RS)对肝星状细胞(HSC)增殖和胶原合成的促进作用.方法采用KCCM、单纯NBS及混合血清刺激大鼠HSC-T6细胞,用3H-TdR和3H-脯氨酸掺入法检测HSC增殖活性和胶原合成状况.结果不同刺激剂作用48 h后,HSC均体积增大,胞突增多延长,核分裂像增多.48 h的3H-TdR和96 h的3H-脯氨酸掺入明显增加.结论混合血清对HSC-T6的综合激活作用较KCCM和单纯10% NBS的作用明显,能明显促进其增殖和胶原合成,是有效的肝纤维化体外模型.
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| 关 键 词： | 枯否细胞 肝/细胞学 培养基 条件性 |
| 文章编号： | 1000-1492(2004)01-0009-04 |
Activation of hepatic stellate cell strain HSC-T6 by Kupffer cell-conditioned medium and mixed sera |
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| Wu Qiang,Yang Yan,Zhang Xiaoxiang et al.Activation of hepatic stellate cell strain HSC-T6 by Kupffer cell-conditioned medium and mixed sera[J].Acta Universitis Medicinalis Anhui,2004,39(1):9-12. |
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| Authors: | Wu Qiang Yang Yan Zhang Xiaoxiang |
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| Abstract: | Objective To study the activation of hepatic stellate cells (HSC) by Kupffer cell-conditioned medium (KCCM), new bovine serum (NBS) and rat serum. Methods Rat HSC-T6 cells were cultured in DMEM with or without KCCM, NBS and normal rat serum. HSC proliferation and collagen secretion were measured with 3H-TdR and 3H-proline incorporation. HSC morphology was observed under light microscope. Results After culturing with KCCM and sera, HSCs enlarged with protrudes elongating and increasing, and mitotic figures increased. 3H-TdR uptake at 48 h and 3H-proline uptake at 96 h were both increased remarkably in a dose-dependent manner. Conclusion Ten percent of new bovine serum together with normal rat serum and KCCM may activate HSC-T6 effectively, accelerating both its DNA and collagen syntheses, which may serve as a model for the study of hepatic fibrosis. |
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| Keywords: | Kupffer cells liver/cytology culture medum conditioned |
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