子宫肌瘤组织雌、孕激素受体和表皮生长因子受体的研究

目的 :探讨雌、孕激素和表皮生长因子与子宫肌瘤生长的关系。方法 :应用免疫组化ABC法检测 4 0例子宫肌瘤患者子宫组织雌激素受体 (ER)、孕激素受体 (PR)和表皮生长因子受体(EGFR)的表达 ,同时用磁性微粒载体免疫酶联法 (IEMA)测定血清雌二醇 (E2 )和孕酮 (P)水平 ;用酶联免疫吸附法 (EL ISA)测定血清表皮生长因子 (EGF)水平。结果 :子宫肌瘤组织 ER、EGFR表达显著高于子宫肌层组织 (P<0 .0 5 ,P<0 .0 1) ,子宫肌瘤组织 PR表达显著高于 ER表达 (P<0 .0 0 5 ) ,肌瘤组织和肌层组织分泌期 EGFR表达显著高于增生期 (P<0 .0 5 )。子宫肌瘤组分泌期血清 E2 、P水平显著高于对照组 (P<0 .0 5 ,P<0 .0 0 1) ,肌瘤组和对照组分泌期 EGF水平均明显高于增生期 (P<0 .0 0 1)。结论 :子宫肌瘤的生长与血清 E2 、P水平升高和子宫组织 ER、PR和 EGFR的表达增强有关。P可能诱导 EGF的分泌和 EGFR的表达

A STUDY ON RECEPTORS OF ESTROGEN, PROGESTERONE AND EPIDERMAL GROWTH FACTOR IN UTERINE LEIOMYOMA

Objective:To study the relationship between estrogen, progesterone, epidermal growth factor (EGF) with uterine leiomyoma.Methods:Expressions of estrogen receptor (ER), progesterone reccptor (PR) and epidermal growth factor receptor (EGFR) in uterine leiomyoma and myometrium were determined by immunohistochemical ABC method in cases with uterine leiomyoma. Serum estradiol (E2) and progesterone ( P ) levels were measured by immunoenxzymetric assay ( IEMA ) and EGF level by enzyme linked immunosorbent assay (ELISA). Results: Expressions of ER and EGFR in uterine leiomyoma were significantly higher than those in myometrium ( P <0.05, P < 0.01 ) . PR expression in uterine leiomyoma was significantly higher than ER expression ( P < 0.005). Expression of EGFR in uterine leiomyoma and myometrium in secretive phase was significantly higher than that in proliferative phase ( P <0.05). Serum E 2 and P levels in leiomyoma group were significantly higher than those in control group during secretive phase ( P <0.05, P <0.001).Serum EGF level in both leiomyoma group and control group in secretive phase was significantly higher than that in proliferative phase ( P <0.001).Conclusion:The growth of uterine leiomyoma is associated with high level of serum E2, P and with strong expression of ER, PR, EGFR in uterine tissuse. It is possible that EGF secretion and EGFR expression may be stimulated by P.