p16、p15蛋白在小儿急性淋巴细胞白血病的表达及临床相关性研究

目的：探讨p16、p15蛋白在急性淋巴细胞白血病（ALL）发病中的意义。方法：对23例ALL细胞进行间接免疫荧光染色，用流式细胞仪检测细胞的荧光强度，间接反映p16、p15蛋白水平。结果：23例ALL患儿p16蛋白阴性10例，p15蛋白阴性8例，p16、p15蛋白均阴性6例。3例T－ALL中p16、p15蛋白皆阴性2例，13例Non T-ALL中，p16蛋白阴性6例，p15蛋白阴性5例。高白细胞组的p16、p15蛋白表达阳性率低于非高白细胞组，差异有显著意义（P＜0．05），HR－ALL组p16、p15蛋白阳性表达低于SR－ALL组，差异有显著意义（P＜0．05）。结论：p16、p15蛋白参与了部分急性淋巴细胞白血病的发病，p15、p15蛋白阴性的患者可能预后不良。

Expression of p16 and p15 Proteins in Acute Lymphoblastic Leukemia of Children

Objective:Our aim was to To clarify the roles of p16 and p15 proteins in the genesis of acute lymphoblastic leukemia(ALL).Methods:Twenty-three samples of ALL were studied by the method of indirect immunofluorescence.Flow cytometer was used to estimate the cellular fluorescent intensity to determine the levels of p16 and p15 proteins.Results:Negative expression for p16 protein was found in 10 of 23 samples,and 8 of 23 were p15 negative expression.Both kinds of proteins were abscent in 6 samples.2 of 3 cases of T-ALL were negative expression of p16,p15 protein.In non T-ALL,6 of 13 were negative expression for p16 protein,5 of 13 were p15 protein deficient.The expression rates of p16,p15 protein in high leukocyte group were lower than those of non-high leukocyte group(P<0.05).The expression rates of p16,p15 proteins in HR-ALL were lower than those of SR-ALL(P<0.05).Conclusion:The p16 and p15 proteins take part in the genesis of ALL.Negative expression of p16,p15 proteins might imply the poor clinical outcome.