黑曲霉酸性β-甘露聚糖酶的发酵工艺

从数十株实验室保藏和土壤分离的黑曲霉菌株中 ,通过筛选和物理化学诱变 ,获得了 1株黑曲霉酸性 β 甘露聚糖酶高产菌株 AspergillusnigerL 76 1。经正交试验选出的最佳产酶培养基为 :魔芋粉 4 0 % ,豆饼粉 2 5 % ,玉米浆 1 5 % ,CaCl2 1 0 % ,KH2 PO4 0 1% ,Na2 HPO40 1% ,MgSO4 ·7H2 O 0 1% ,pH6 0。优化的发酵条件为 :装液量 30mL/2 5 0mL三角瓶 ,摇床转速2 2 5r/min ,发酵温度和时间分别为 ( 31± 1)℃和 84h。在上述条件下 ,L 76 1的 β 甘露聚糖酶发酵酶活力达 32 0U/mL。

Fermentation Process of Acidic β-Mannanase from Aspergillus niger

Acidic β-mannanase was effectively produced by Aspergillus niger L-76-1 isolated from laboratory and wild strains through selection,and physical and chemical mutagenesis.The ingredient of shake flask medium determined by orthogonal experiment was composed of 4.0% konjak powder,2.5% soybean meal,1.5% steep liquor,1.0% CaCl 2,0.1% KH 2PO 4,0.1% Na 2HPO 4,0.1 MgSO 4·7H 2O,and initial pH6.0.The optimal cultural conditions for acidic β-mannanase production were as follows:medium volume 30?mL/250?mL shake flask,rotational speed 225r/min,cultural temperature and time 31±1℃ and 84?h respectively.With these conditions,the acidic β-mannanase yielded by L-76-1 reached 320?U/mL.