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| 菜用大豆高效胚尖离体再生基因型筛选 | |
| 引用本文： | 钟灿,肖深根,朱保葛,薛仁镐,卫志明,朱木兰.菜用大豆高效胚尖离体再生基因型筛选[J].大豆科学,2012,31(1):9-12. |
| 作者姓名： | 钟灿 肖深根 朱保葛 薛仁镐 卫志明 朱木兰 |
| 作者单位： | 1. 湖南农业大学园艺园林学院,湖南长沙,410128 2. 中国科学院遗传与发育生物学研究所,北京,100101 3. 青岛农业大学生命科学学院,山东青岛,266109 4. 中国科学院上海生命科学研究院,植物生理生态研究所,国家植物基因研究中心(上海),上海200032 |
| 基金项目： | 转基因生物新品种培育重大专项资助项目(2009ZX08010-009B,2009ZX08004-009B) |
| 摘 要： | 以华东地区4个主栽菜用大豆品种(交大05-133、交大02-89、沪宁96-10、青酥二号)的胚尖为起始外植体,研究消毒方法、预培养天数、6-BA浓度和培养基组合等对不定芽的诱导和伸长的影响。结果表明:用0.1%HgCl2消毒10 min后配合5%的NaClO消毒5 min,消毒效果最佳,胚尖活力好,且适用于各个品种;预培养时间为2 d,6-BA浓度为3.0 mg.L-1,有利于菜用大豆不定芽的诱导;1.0 mg.L-16-BA+0.1 mg.L-1NAA培养基组合有利于增加有效不定芽数;0.05 mg.L-16-BA+0.1 mg.L-1IBA培养基组合有利于不定芽的伸长;交大05-133为最佳胚尖离体再生基因型,其分化频率为90.86%,诱导15 d后外植体平均不定芽数为4.65个,不定芽平均长度为1.38 cm。 |
| 关 键 词： | 菜用大豆 胚尖 基因型筛选 离体再生 |
Selection of High-efficient Regeneration Genotype from Embryonic Tips of Vegetable-type Soybean |
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| ZHONG Can , XIAO Shen-gen , ZHU Bao-ge , XUE Ren-gao , WEI Zhi-ming , ZHU Mu-lan.Selection of High-efficient Regeneration Genotype from Embryonic Tips of Vegetable-type Soybean[J].Soybean Science,2012,31(1):9-12. | |
| Authors: | ZHONG Can XIAO Shen-gen ZHU Bao-ge XUE Ren-gao WEI Zhi-ming ZHU Mu-lan |
| Affiliation: | 1.College of Horticulture and Gardening,Hunan Agricultural University,Changsha 410128,Hunan;2.Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,National Center for Plant Gene Research(Shanghai)Chinese Academy of Sciences(CAS),Shanghai 200032;3.Institute of Genetics and Developmental Biology,CAS,Beijing 100101;4.College of Life and Science,Qingdao Agricultural University,Qingdao 266109,Shandong,China) |
| Abstract: | To study the effect of sterilization,pre-culture days,different concentrations of 6-BA and medium formulations on induction and elongation of adventitious buds,embryonic tips from four vegetable-type soybean varieties ’Jiaoda 05-133’,’Jiaoda 02-89’,’Huning 96-10’ and ’Qingsuerhao’ were used as the initial explants.The results showed that the optimal sterilization was using 0.1% HgCl2 for 10 min and 5% NaClO for 5 min.The optimal adventitious buds induction formulation was MSB5 base medium with 3.0 mg·L-1 6-BA,and the optimal pre-culture days of embryonic tips was 2 d.The combination of 1.0 mg·L-1 6-BA and 0.1 mg·L-1 NAA benefited to produce many adventitious buds.The combination of 0.05 mg·L-1 6-BA and 0.1 mg·L-1 IBA benefited to the elongation of adventitious buds.Among the four varieties,the embryonic tips from Jiaoda 05-133 is the best one for regeneration,with 90.86% of adventitious buds induction rate,4.65 of mean number of adventitious buds and 1.38 cm of mean length of adventitious buds after culture for 15 d. |
| Keywords: | Vegetable-type soybean Embryonic tips Selection of genotype Regeneration |
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